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Neues Enzym mit Reverse Transkriptaseund DNA-Polymerase-Funktion

  • Anwendungen & Produkte
  • Reverse Transkriptase-Polymerase-Kettenreaktion (RT-PCR)
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BIOspektrum Aims and scope

Abstract

Reverse transcriptions under high temperatures can be performed with a new designed, thermostable DNA polymerase. This DNA polymerase was generated from Taq DNA polymerase by directed evolution in multiple rounds of mutagenesis and screening. The new DNA polymerase combines an artificially induced reverse transcriptase activity with the natural thermostability of Taq DNA polymerase. Potential applications are depicted here.

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Literatur

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Authors and Affiliations

  1. myPOLS GmbH, c/o Universität Konstanz, Fach 726, Universitätsstraße 10, D-78457, Konstanz, Deutschland

    Ramon Kranaster, Jessica Zeller & Andreas Marx

  2. Lehrstuhl für Organische Chemie/Zelluläre Chemie Fachbereich Chemie, Fach M 726, Universität Konstanz, D-78457, Konstanz, Deutschland

    Birgit Köhn & Andreas Marx

Authors
  1. Ramon Kranaster
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  2. Jessica Zeller
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  3. Birgit Köhn
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  4. Andreas Marx
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Corresponding authors

Correspondence to Ramon Kranaster or Andreas Marx.

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Kranaster, R., Zeller, J., Köhn, B. et al. Neues Enzym mit Reverse Transkriptaseund DNA-Polymerase-Funktion. Biospektrum 22, 164–165 (2016). https://doi.org/10.1007/s12268-016-0671-6

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  • DOI: https://doi.org/10.1007/s12268-016-0671-6

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