BIOspektrum

, Volume 22, Issue 1, pp 62–64 | Cite as

CRISPR in der biotechnologischen Forschung und Entwicklung

  • Ümit Pul
  • Jörg Mampel
  • Christian Zurek
  • Michael Krohn
Anwendungen & Produkte Genomeditierung

Abstract

The discovery of programmable double-stranded DNA specific nucleases derived from the prokaryotic immunity systems CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) has opened a new era of genome editing and its applications in industrial biotechnology. Simple reprogramming of the DNA-specificity of CRISPR nucleases by RNAs allows the engineering of novel eukaryotic screening or producer cells through gene knock-out, chromosomal deletion and the regulation of gene expression.

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Literatur

  1. [1]
    Sorek R, Lawrence CM, Wiedenheft B (2013) CRISPRmediated adaptive immune systems in bacteria and archaea. Annu Rev Biochem 82:237–266CrossRefPubMedGoogle Scholar
  2. [2]
    Doudna JA, Charpentier E (2014) Genome editing. The new frontier of genome engineering with CRISPR-Cas9. Science 346:1258096CrossRefPubMedGoogle Scholar
  3. [3]
    Jinek M, Chylinski K, Fonfara I et al. (2012) A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337:816–821CrossRefPubMedGoogle Scholar
  4. [4]
    Konermann S, Brigham MD, Trevino AE et al. (2015) Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature 517:583–588PubMedCentralCrossRefPubMedGoogle Scholar
  5. [5]
    Gilbert LA, Horlbeck MA, Adamson B et al. (2014) Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation. Cell 159:647–661PubMedCentralCrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Ümit Pul
    • 1
  • Jörg Mampel
    • 1
  • Christian Zurek
    • 1
  • Michael Krohn
    • 1
  1. 1.B.R.A.I.N Aktiengesellschaft, BioActives & Performance BiologicalsZwingenbergDeutschland

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