Abstract
Methanol is an attractive alternative carbon source for industrial biotechnology. Methylobacterium species represent suitable microorganisms to convert methanol into valuable multi-carbon chemicals, as their complex central metabolism provides many different useful starting intermediates for establishing efficient production pathways. Here we describe recent results to produce dicarboxylic acids and terpenoids in M. extorquens.
Literatur
Mac Lennan DG, Gow JS, Stringer DA (1973) The ICI methanol-bacterium process for the production of single-cell protein. Proc Roy Aust Chem Inst 40:54–61
Sonntag F, Buchhaupt M, Schrader J (2014) Thioesterases for ethylmalonyl-CoA pathway derived dicarboxylic acid production in Methylobacterium extorquens AM1. Appl Microbiol Biotechnol 98:4533–4544
Erb TJ, Berg IA, Brecht V et al. (2007) Synthesis of C5- dicarboxylic acids from C2-units involving crotonyl-CoA carboxylase/ reductase: The ethylmalonyl-CoA pathway. Proc Natl Acad Sci 104: 10631–10636
Alber BE (2011) Biotechnological potential of the ethylmalonyl- CoA pathway. Appl Microbiol Biotechnol 89:17–25
Sonntag F, Müller JE, Kiefer P et al. (2015) High-level production of ethylmalonyl-CoA pathway-derived dicarboxylic acids by Methylobacterium extorquens under cobalt-deficient conditions and by polyhydroxybutyrate negative strains. Appl Microbiol Biotechnol 99:3407–3419
Kiefer P, Buchhaupt M, Christen P et al. (2009) Metabolite Profiling Uncovers Plasmid-Induced Cobalt Limitation under Methylotrophic Growth Conditions. PLoS ONE 4:e7831
Sonntag F, Kroner C, Lubuta P et al. (2015) Engineering Methylobacterium extorquens for de novo synthesis of the sesquiterpenoid α-humulene from methanol. Metab Eng, im Druck
Fernandes ES, Passos GF, Medeiros R et al (2007) Antiinflammatory effects of compounds alpha-humulene and (–)-trans-caryophyllene isolated from the essential oil of Cordia verbenacea. Eur J Pharmacol 569:228–236
Kaczmarczyk A, Vorholt JA, Francez-Charlot A (2013) Cumate-inducible gene expression system for sphingomonads and other Alphaproteobacteria. Appl Environ Microbiol 79:6795–6802
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Buchhaupt, M., Schrader, J. Methanol als alternative Kohlenstoffquelle für mikrobielle Produktionsprozesse. Biospektrum 21, 672–674 (2015). https://doi.org/10.1007/s12268-015-0629-0
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12268-015-0629-0