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Automatisierte Herstellung von ChIP-Seq-Sequenzbibliotheken

  • Anwendungen & Produkte
  • Next Generation Sequencing
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Abstract

Manual library preparation for ChIP-Seq applications is very tedious to conduct and lacks process standardization. Here we translate a commercially available ChIP-Seq library preparation protocol into commands for the Eppendorf epMotion automated liquid handling system and show that libraries with comparable quantity and quality can be easily generated, liberating lab personnel from repetitive processing steps while maximizing reproducibility and data comparability.

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Authors and Affiliations

  1. Max-Planck-Institut für Immunbiologie und Epigenetik, Stübeweg 51, D-79108, Freiburg, Germany

    Ulrike Bönisch, Laura Arrigoni, Emily Betancourt & Kerstin Bruder

Authors
  1. Ulrike Bönisch
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  2. Laura Arrigoni
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  3. Emily Betancourt
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  4. Kerstin Bruder
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Corresponding author

Correspondence to Ulrike Bönisch.

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Bönisch, U., Arrigoni, L., Betancourt, E. et al. Automatisierte Herstellung von ChIP-Seq-Sequenzbibliotheken. Biospektrum 21, 526–527 (2015). https://doi.org/10.1007/s12268-015-0612-9

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  • DOI: https://doi.org/10.1007/s12268-015-0612-9

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