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Eine Roboterplattform für das Hochdurchsatz-Screening von Biokatalysatoren

  • Biotechnologie
  • Enzymtechnologie
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BIOspektrum Aims and scope

Abstract

We have established an automated robotic platform to perform all steps required for enzyme production and analysis in a microtiter plate format. This system covers colony picking, growth, protein expression, enzyme isolation and in-depth biochemical characterization using spectrophotometric and fluorimetric assays. It has successfully been applied to screen mutant libraries of biocatalysts for e.g., substrate scope, enantioselectivity or stability for various enzyme classes such as transaminases, Baeyer-Villiger monoxygenases, esterases and dehalogenases.

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Authors and Affiliations

  1. Institut für Biochemie, Abteilung Biotechnologie und Enzymkatalyse, Ernst-Moritz-Arndt-Universität Greifswald, Felix-Hausdorffstraße 4, D-17487, Greifswald, Deutschland

    Mark Dörr, Dominique Böttcher, Anke Hummel, Matthias Höhne & Uwe Bornscheuer

Authors
  1. Mark Dörr
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  2. Dominique Böttcher
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  3. Anke Hummel
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  4. Matthias Höhne
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  5. Uwe Bornscheuer
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Corresponding author

Correspondence to Uwe Bornscheuer.

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Dörr, M., Böttcher, D., Hummel, A. et al. Eine Roboterplattform für das Hochdurchsatz-Screening von Biokatalysatoren. Biospektrum 21, 230–231 (2015). https://doi.org/10.1007/s12268-015-0563-1

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  • DOI: https://doi.org/10.1007/s12268-015-0563-1

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