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Gezielte Anreicherung bakterieller DNA

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Abstract

Cytosine methylation and frequency of non-methylated CpG dinucleotides are genomic properties varying greatly between mammals and bacteria. A new DNA enrichment technology uses the specific affinity of the CXXC zinc finger domain for non-methylated CpG dinucleotides for powerful bacterial nucleic acid separation and enrichment. Application of the technology in sample preparation allows a 50-fold enrichment of minute amounts of bacterial DNA relative to a high mammalian DNA background.

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Authors and Affiliations

  1. Life Science, Analytik Jena AG, Konrad-Zuse-Straße 1, D-07745, Jena, Deutschland

    Dirk Osterloh, Susann Motzke & Martina Pick

Authors
  1. Dirk Osterloh
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  2. Susann Motzke
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  3. Martina Pick
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Correspondence to Dirk Osterloh.

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Osterloh, D., Motzke, S. & Pick, M. Gezielte Anreicherung bakterieller DNA. Biospektrum 20, 780–781 (2014). https://doi.org/10.1007/s12268-014-0520-4

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  • DOI: https://doi.org/10.1007/s12268-014-0520-4

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