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Koordinatenbestimmung von Proteinpunkten in 2D-PAGE-Gelen

  • Anwendungen & Produkte
  • 2D-Gelelektrophorese
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BIOspektrum Aims and scope

Abstract

A major problem in two-dimensional protein gel electrophoresis is the little comparability of gel images. A reference grid of marker proteins in parallel to the separation of the analyte by use of two fluorescent dyes allows the correction of protein coordinates. The deviation from mean is improved by an order of magnitude. The technology called comparative 2D fluorescence gel electrophoresis (CoFGE) can be carried out in both vertical and horizontal electrophoresis instrumentation.

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Literatur

  1. Ackermann D, Wang W, Streipert B et al. (2012) Comparative fluorescence two-dimensional gel electrophoresis using a gel strip sandwich assembly for the simultaneous ongel generation of a reference protein spot grid. Electrophoresis 33:1406–1410

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Authors and Affiliations

  1. Integrierte Funktionelle Genomik, Interdisziplinäres Zentrum für Klinische Forschung, Universität Münster, Röntgenstraße 21, D-48149, Münster, Deutschland

    Marina Hanneken, Doreen Ackermann & Simone König

  2. SERVA Electrophoresis GmbH, Carl-Benz-Straße 7, D-69115, Heidelberg, Deutschland

    Günter Thesseling

Authors
  1. Marina Hanneken
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  2. Doreen Ackermann
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  3. Simone König
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  4. Günter Thesseling
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Corresponding authors

Correspondence to Simone König or Günter Thesseling.

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Hanneken, M., Ackermann, D., König, S. et al. Koordinatenbestimmung von Proteinpunkten in 2D-PAGE-Gelen. Biospektrum 20, 655–657 (2014). https://doi.org/10.1007/s12268-014-0503-5

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  • DOI: https://doi.org/10.1007/s12268-014-0503-5

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