Membranproteinsynthese: Zellfrei geht’s schneller!
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Difficult to express membrane proteins represent an increasing amount of therapeutic molecules. Considerable optimization is often required for downstream applications such as assay development and functional characterization. Cell-free systems emerged as powerful tools for the synthesis of structurally and functionally divergent membrane proteins. Vesicle-based eukaryotic cell-free systems enable co-translational protein translocation and posttranslational modifications. Hence, these systems provide a multitude of options for membrane protein studies.
- Kubick S, Gerrits M, Merk H et al. (2009) In vitro synthesis of posttranslationally modified membrane proteins, In: DeLucas L (Hrsg) Membrane Protein Crystallization. Current Topics in Membranes, Academic Press, Elsevier, San DiegoGoogle Scholar