, Volume 20, Issue 4, pp 453–455 | Cite as

3D-Epoxyoberflächen für DNA-codierte Proteinarrays

  • Oda Stoevesandt
  • Ronny Schmidt
  • Christian Heise
  • Uwe SchedlerEmail author
Biotechnologie Trägermaterialien


The DNA-array to protein-array technology (DAPA) allows the direct transcription and translation of a coded DNA-array to a protein array in the presence of a cell free expression system. The coupling efficiency of DNA and of the corresponding immobilized proteins is enhanced by using 3-dimensional epoxy surfaces. The production time of protein arrays is considerably reduced and the DNA template array can be reused for producing further protein arrays.


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  1. [1]
    Stoevesandt O, Taussig MJ, He M (2009) Protein microarrays: high throughput tools for proteomics. Expert Rev Proteomics 6:145–157PubMedCrossRefGoogle Scholar
  2. [2]
    Jeong JS, Jiang L, Albino E et al. (2012) Rapid identification of monospecific monoclonal antibodies using a human proteome microarray. Mol Cell Proteomics 11:O111.016253Google Scholar
  3. [3]
    Hu CJ, Song G, Huang W et al. (2012) Identification of new autoantigens for primary biliary cirrhosis using human proteome microarrays. Mol Cell Proteomics 11:669–680PubMedCentralPubMedCrossRefGoogle Scholar
  4. [4]
    He M, et al. (2008) Printing protein arrays from DNA arrays. Nat Methods 5:175–177PubMedCrossRefGoogle Scholar
  5. [5]
    Stoevesandt O (2012) Protein arraying by cell-free expression and diffusion across a fluid-filled gap. N Biotechnol 29:586–588PubMedCrossRefGoogle Scholar
  6. [6]
    Schmidt R, et al. (2013) Optimised ‘on demand’ protein arraying from DNA by cell free expression with the ‘DNA to Protein Array’ (DAPA) technology. J Proteomics 88:141–148PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Oda Stoevesandt
    • 1
  • Ronny Schmidt
    • 1
  • Christian Heise
    • 2
  • Uwe Schedler
    • 2
    • 3
    Email author
  1. 1.Cambridge Protein Arrays Ltd.CambridgeUK
  2. 2.Polyan GmbHBerlinGermany
  3. 3.PolyAn GmbHBerlinGermany

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