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BIOspektrum

, Volume 20, Issue 4, pp 416–418 | Cite as

Gewinnung haploider Stammzellkulturen der Maus für genetische Screens

  • Remo FreimannEmail author
  • Stefan Kramer
  • Andreas BöhmlerEmail author
  • Anton Wutz
Anwendungen & Produkte Stammzellen

Abstract

Haploid mouse embryonic stem cells are a handy tool to investigate loss of function mutations. Furthermore, these cells can contribute to chimeric embryos and can be used to introduce genetic modifications into mouse germ lines. Nevertheless, haploid cell cultures have to be maintained by flow sorting on a regular basis due to spontaneous diploidization. Here we present physical differences between haploid and diploid cells, which can be used in applied cell sorting of haploid cell cultures.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Institut für Molekulare GesundheitswissenschaftenEth ZürichSchweizGermany
  2. 2.Beckman Coulter GmbhKrefeldGermany
  3. 3.Institut für Molekulare GesundheitswissenschaftenETH ZürichZürichGermany
  4. 4.Beckman Coulter GmbHKrefeldGermany

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