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Gewinnung haploider Stammzellkulturen der Maus für genetische Screens

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Abstract

Haploid mouse embryonic stem cells are a handy tool to investigate loss of function mutations. Furthermore, these cells can contribute to chimeric embryos and can be used to introduce genetic modifications into mouse germ lines. Nevertheless, haploid cell cultures have to be maintained by flow sorting on a regular basis due to spontaneous diploidization. Here we present physical differences between haploid and diploid cells, which can be used in applied cell sorting of haploid cell cultures.

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Authors and Affiliations

  1. Institut für Molekulare Gesundheitswissenschaften, Eth Zürich, Schweiz, Germany

    Remo Freimann, Stefan Kramer & Anton Wutz

  2. Beckman Coulter Gmbh, Krefeld, Germany

    Andreas Böhmler

  3. Institut für Molekulare Gesundheitswissenschaften, ETH Zürich, Otto-Stern-Weg 7, CH-8093, Zürich, Germany

    Remo Freimann

  4. Beckman Coulter GmbH, Europark Fichtenhain B13, D-47807, Krefeld, Germany

    Andreas Böhmler

Authors
  1. Remo Freimann
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  2. Stefan Kramer
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  3. Andreas Böhmler
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  4. Anton Wutz
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Corresponding authors

Correspondence to Remo Freimann or Andreas Böhmler.

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Freimann, R., Kramer, S., Böhmler, A. et al. Gewinnung haploider Stammzellkulturen der Maus für genetische Screens. Biospektrum 20, 416–418 (2014). https://doi.org/10.1007/s12268-014-0458-6

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  • DOI: https://doi.org/10.1007/s12268-014-0458-6

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