Skip to main content
SpringerLink
Log in

EdU statt BrdU: eine Alternative zur Detektion der Zellproliferation

DNA-Synthese-Nachweis

  • Anwendungen & Produkte
  • Published:
BIOspektrum Aims and scope

Abstract

The use of BrdU (5′-bromo-2′-deoxyuridine) is a widely accepted method for the detection of cell proliferation. Despite the good performance, this method has yet shown some disadvantages such as the destruction of the target DNA in order to allow the antibody-driven colorimetric/fluorescent detection of newly synthesized cells. This harsh DNA destructing attack on the system can be overcome by using EdU (5′-ethynyl-2′-deoxyuridine) as innovative method based on click chemistry.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  1. Salic A, Mitchison TJ (2008) A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc Natl Acad Sci USA 105:2415–2420

    Article  PubMed Central  PubMed  Google Scholar 

  2. Chehrehasa F, Meedeniya AC, Dwyer P et al. (2009) EdU, a new thymidine analogue for labeling proliferating cells in the nervous system. J Neurosci Methods 177:122–130

    Article  PubMed  CAS  Google Scholar 

  3. Halnes KM, Feldman EL, Lentz SI (2010) Visualization of mitochondrial DNA replication in individual cells by EdU signal amplification. J Vis Exp 45:e2147

    Google Scholar 

  4. Diermeier-Daucher S, Clarke ST, Hill D et al. (2009) Cell type specific applicability of 5-ethynyl-20-deoxyuridine (EdU) for dynamic proliferation assessment in flow cytometry. Cytometry 75A:535–546

    Article  CAS  Google Scholar 

  5. Yu Y, Arora A, Min W et al. (2009) EdU incorporation is an alternative non-radioactive assay to [3H]thymidine uptake for in vitro measurement of mice T-cell proliferations. J Immunol Methods 350:29–35

    Article  PubMed  CAS  Google Scholar 

  6. Rostovtsev VV, Green LG, Fokin VV et al. (2002) Stepwise huisgen cycloaddition process: copper(I)-catalyzed regioselective „ligation“ of azides and terminal alkynes. Angew Chem Int Ed 41:2596–2599

    Article  CAS  Google Scholar 

  7. Tornøe CW, Christensen C, Meldal M (2002) Peptidotriazoles on solid phase: [1,2,3]-triazoles by regiospecific copper(I)-catalyzed 1,3-dipolar cycloadditions of terminal alkynes to azides. J Org Chem 67:3057–3064

    Article  PubMed  CAS  Google Scholar 

  8. Gramlich PM, Wirges CT, Manetto A et al. (2008) Postsynthetic DNA modification through the copper-catalyzed azide-alkyne cycloaddition reaction. Angew Chem Int Ed 47:8350–8358

    Article  CAS  Google Scholar 

  9. Salic A, Mitchison TJ (2008) A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc Natl Acad Sci USA 105:2415–2420

    Article  PubMed Central  PubMed  Google Scholar 

  10. Dorsett M, Westlund B, Schedl T (2009) METT-10, a putative methyltransferase, inhibits germ cell proliferative fate in Caenorhabditis elegans. Genetics 183:233–247

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  11. Bando T, Mito T, Maeda Y et al. (2009) Regulation of leg size and shape by the Dachsous/Fat signalling pathway during regeneration. Development 136:2235–2245

    Article  PubMed  CAS  Google Scholar 

  12. Kaiser CL, Kamien AJ, Shah PA (2009) EdU labeling detects proliferating cells in the regenerating avian cochlea. Laryngoscope 119:1770–1775

    Article  PubMed Central  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Baseclick GmbH, Tutzing, Germany

    Birgit Oberleitner, Antonio Manetto & Thomas Frischmuth

  2. baseclick GmbH, Bahnhofstraße 9-15, D-82327, Tutzing, Germany

    Antonio Manetto

Authors
  1. Birgit Oberleitner
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Antonio Manetto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Thomas Frischmuth
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Antonio Manetto.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Oberleitner, B., Manetto, A. & Frischmuth, T. EdU statt BrdU: eine Alternative zur Detektion der Zellproliferation. Biospektrum 20, 188–190 (2014). https://doi.org/10.1007/s12268-014-0428-z

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12268-014-0428-z

Search

Navigation