Abstract
Flow cytometry is by far the most sophisticated and accurate method for sorting of living stem cells. In general, the target cells need to be labeled for various cell identity markers, which are exposed on the surface of the cells. However, in non-model organisms, we usually lack specific labels for such cell surface markers. Here we describe a method for isolating stem cells from planarians with flow cytometry, based on physiological and morphological properties of these cells.
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Martin Stehling Jahrgang 1971. Biologiestudium an der Universität Münster. 2001 Promotion, 2002–2004 Mitarbeiter bei der Partec GmbH, Münster. Seit 2004 Zentrale Einheit für Durchflusszytometrie am Max-Planck-Institut für molekulare Biomedizin in Münster.
Henning Schmitz Jahrgang 1982. 2007–2010 Bachelor-Studium Biowissenschaften an der Universität Münster. 2011–2013 Master-Studium Biowissenschaften mit Schwerpunkt Molekulare Biomedizin an der Universität Münster. Seit 2014 PhD-Studium Biologie am Max-Planck-Institut für molekulare Biomedizin in Münster.
Luca Gentile Jahrgang 1975. 1995-2000 Biologiestudium an der Universität Pavia, Italien. 2004 Promotion (PhD) an der Universität Pavia. 2004–2010 Postdoc am Max-Planck-Institut für molekulare Biomedizin in Münster. Seit 2012 Projektleiter des Planarian Stem Cell Laboratory am Max-Planck-Institut für molekulare Biomedizin in Münster.
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Schmitz, H., Stehling, M. & Gentile, L. Selbstheilung und potenzielle Unsterblichkeit bei Planarien. Biospektrum 20, 51–54 (2014). https://doi.org/10.1007/s12268-014-0408-3
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DOI: https://doi.org/10.1007/s12268-014-0408-3