Abstract
Reliable and sensitive single target copy detection, as well as selection of reliable reference genes for precise gene expression analysis, are still challenges for real-time PCR users. We introduce a system that enables robust and highly sensitive probe-based real-time PCR, provides a novel, antibody-mediated, hot-start mechanism for highly specific target detection, and allows flexible combinations of high and low abundant target and reference genes for accurate gene expression results.
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Irle, I., Traeger, T. & Missel, A. Akkurate Detektion seltener Gene und flexible Auswahl der Referenzgene. Biospektrum 19, 766–767 (2013). https://doi.org/10.1007/s12268-013-0390-1
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DOI: https://doi.org/10.1007/s12268-013-0390-1