Schnelle und aussagekräftige Genotoxizitätsbewertung

Abstract

At present, just a few of the available in vitro genotoxicity tests measure DNA strand breaks and their repair in cells. The comet assay as the considered “gold standard” is still not standardized and does not allow full automation so far. In contrast, most steps of the fluorimetic analysis of alkaline DNA unwinding (FADU) assay could be automated which allows precise dispensing of solutions and control of temperature and process time during the assay. This progress has led to improved reproducibility and throughput, easier handling, and enhanced sensitivity and speed.

This is a preview of subscription content, log in to check access.

Literatur

  1. [1]

    Krebs JE, Goldstein ES, Kilpatrick ST (2012) Lewin’s Genes XI. Jones & Bartlett, Burlington

    Google Scholar 

  2. [2]

    Rovida C, Hartung T (2009) Re-evaluation of animal numbers and costs for in vivo tests to accomplish REACH legislation requirements for chemicals — a report by the transatlantic think tank for toxicology (t(4)). ALTEX 26:187–208

    PubMed  Google Scholar 

  3. [3]

    The Organisation for Economic Co-operation and Development (OECD): Richtlinien zur toxikologischen Prüfungen von Chemikalien. www.bfr.bund.de/de/oecd_richtlinien_zur_toxikologischen_pruefungen_von_chemikalien-61575.html

  4. [4]

    Ostling O, Johanson KJ (1984) Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem Biophys Res Commun 132:291–298

    Article  Google Scholar 

  5. [5]

    Olive PL, Banáth JP (2006) The comet assay: a method to measure DNA damage in individual cells. Nat Protoc 1:23–29

    PubMed  Article  CAS  Google Scholar 

  6. [6]

    Birnboim HC, Jevcak JJ (1981) Fluorometric method for rapid detection of DNA strand breaks in human white blood cells produced by low doses of radiation. Cancer Res 41:1889–1892

    PubMed  CAS  Google Scholar 

  7. [7]

    Moreno-Villanueva M, Pfeiffer R, Sindlinger T et al. (2009) A modified and automated version of the ‘Fluorometric Detection of Alkaline DNA Unwinding’ method to quantify formation and repair of DNA strand breaks. BMC Biotechnology 9:39 (doi: 10.1186/1472-6750-9-39)

    PubMed  Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Marcel Pilartz.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Moreno-Villanueva, M., Pilartz, M. & Unger, M. Schnelle und aussagekräftige Genotoxizitätsbewertung. Biospektrum 19, 536–538 (2013). https://doi.org/10.1007/s12268-013-0352-7

Download citation