Analyse eines unkonventionellen Vitamin-Transporters


Förster resonance energy transfer (FRET) identifies the co-localization of fluorescent macromolecules on a nanometer scale. FRET between nonidentical and identical fluorophores is called HeteroFRET or HomoFRET, respectively. We applied FRET techniques to analyze the oligomeric state of the core component (BioY) of a bacterial biotin transporter. BioY was fused to fluorescent proteins suitable for Hetero- and HomoFRET analyses. Spectrometric and imaging experiments with living cells strongly support a model with oligomers of BioY as the functional state of the core unit.

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Correspondence to Joanna Ziomkowska or Franziska Kirsch or Andreas Herrmann or Thomas Eitinger.

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Franziska Kirsch, Thomas Eitinger, Joanna Ziomkowska und Andreas Herrmann (v. l. n. r.)

Die Arbeitsgruppe von Andreas Herrmann beschäftigt sich mit Lipid- und Protein-Wechselwirkungen und deren Bedeutung für die Struktur und Dynamik von Membranen. Schwerpunkte in der Arbeitsgruppe von Thomas Eitinger bilden bakterielle Aufnahmesysteme, unter anderem verschiedene Vertreter der ECF-Transporter für Vitamine und Übergangsmetallionen.

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Ziomkowska, J., Kirsch, F., Herrmann, A. et al. Analyse eines unkonventionellen Vitamin-Transporters. Biospektrum 18, 493–496 (2012).

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