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High-content-Analyse von 3D-Zellkulturen

Medikamentenentwicklung

  • Biotechnologie
  • Published:
BIOspektrum Aims and scope

Abstract

Techniques like the hanging-drop method allow cells to form organotypic microtissues. To apply more complex three-dimensional cell model systems for drug discovery, optical analysis technologies are mandatory to assess the biological response of substances. In this study we describe the analysis of a spherical colon cancer microtissue model with a high content analysis platform. Beside the analysis of microtissue growth, a far red emitting activatable fluorescence dye indicating hypoxic areas in tissues was used.

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Author information

Authors and Affiliations

  1. Perkinelmer, Hamburg, Germany

    Marcel Waschow & Karin Böttcher

  2. Insphero AG, Zürich, Germany

    Maren Drewitz & Jens M. Kelm

  3. InSphero AG, Technoparkstraße 1, CH-8005, Zürich, Germany

    Karin Böttcher

  4. PerkinElmer Cellular Technologies Germany GmbH, Schnackenburgallee 114, D-22525, Hamburg, Germany

    Jens M. Kelm

Authors
  1. Marcel Waschow
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  2. Maren Drewitz
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  3. Karin Böttcher
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  4. Jens M. Kelm
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Corresponding authors

Correspondence to Karin Böttcher or Jens M. Kelm.

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Waschow, M., Drewitz, M., Böttcher, K. et al. High-content-Analyse von 3D-Zellkulturen. Biospektrum 18, 447–448 (2012). https://doi.org/10.1007/s12268-012-0206-8

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  • DOI: https://doi.org/10.1007/s12268-012-0206-8

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