Abstract
Numerous applications in biotechnology would not be feasible without optimized enzymes. While changing the substrate specificity, enzyme libraries with mutations near the substrate binding pocket give often impressive results. Besides elaborated methods for preparing such libraries, efficient screening procedures are also crucial for project success. An example on library design and screening using new technologies will be given based on the engineering of an aspartase.
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Greiner-Stöffele T, Feller C, Struhalla M (2009) Verfahren zur Erzeugung einer Varianten-Bibliothek von DNASequenzen. EP2130918A1 & WO2009146892A1
Greiner-Stöffele T, Struhalla M (2004) Verfahren zur Selektion von Biomolekülen aus Varianten-Bibliotheken von Biomolekülen. DE10350474B4 & WO2005040376A3
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Thomas Greiner-Stöffele 1992–1997 Biochemiestudium an der Universität Leipzig. 2000 Promotion bei Prof. Dr. U. Hahn, Universität Leipzig. 2000-2001 Postdoc bei Prof. Dr. M. Eigen, Max-Planck-Institut für Biophysikalische Chemie, Göttingen. 2001 Mitarbeiter Direvo AG, Köln. 2001–2008 Nachwuchsgrup pen leiter an der Universität Leipzig. Seit 2004 Mitgründer und wissenschaftlicher Leiter der c-LEcta GmbH, Leipzig.
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Greiner-Stöffele, T. Optimierte Enzyme mittels fokussierter Bibliotheken und Cluster-Screening. Biospektrum 18, 380–381 (2012). https://doi.org/10.1007/s12268-012-0196-6
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DOI: https://doi.org/10.1007/s12268-012-0196-6