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Detektion der Side Population zur Identifizierung von Stammzellen

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Abstract

The quantification of the cellular transport capacity for the DNA staining dye Hoechst 33342 out of the cell is a widely accepted marker for recognition of stem cells. Cells with such an increased level of dye efflux (the so-called side population) can be detected on a flow cytometer equipped with a UV laser. Active transport mechanisms of this type play an important role for the elimination of anti-proliferative agents by cancer cells. A staining method for cells with high dye efflux is decribed.

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Authors and Affiliations

  1. Partec GMBH, Münster, Germany

    Danny Köhler & Wolfgang Göhde

  2. Klinik und Poliklinik Für Strahlentherapie — Radioonkologie, Universitätsklinik Münster, Münster, Germany

    Burkhard Greve

  3. Medizinische Fakultät, Westfälische Wilhelms-Universität Münster, Münster, Germany

    Wolfgang Göhde

  4. Partec GmbH, Otto-Hahn-Straße 32, D-48161, Münster, Germany

    Danny Köhler

Authors
  1. Danny Köhler
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  2. Burkhard Greve
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  3. Wolfgang Göhde
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Corresponding author

Correspondence to Danny Köhler.

Additional information

Danny Köhler, Burkhard Greve und Wolfgang Göhde (v. l. n. r.)

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Köhler, D., Greve, B. & Göhde, W. Detektion der Side Population zur Identifizierung von Stammzellen. Biospektrum 18, 58–59 (2012). https://doi.org/10.1007/s12268-012-0144-5

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  • DOI: https://doi.org/10.1007/s12268-012-0144-5

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