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Die Real-Time-RT-PCR-Datenanalyse im Fokus der MIQE-Richtlinie

  • Evaluierung der qPCR
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BIOspektrum Aims and scope

Zusammenfassung

Die MIQE-Richtlinie wurde 2009 von einer Gruppe internationaler Wissenschaftler ins Leben gerufen, um die Qualität, die Richtigkeit sowie die Zuverlässigkeit der gewonnenen qPCR-Ergebnisse im Labor und in der wissenschaftlichen Literatur zu steigern.

Abstract

The MIQE guidelines were established 2009 by a group of international scientist to improve the quality, the accuracy, and the reliability of the generated quantitative PCR results in the lab and in the scientific literature.

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Literatur

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Authors and Affiliations

  1. Lehrstuhl für Physiologie, Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung & Umwelt, TU München, München, Germany

    Michael W. Pfaffl & Irmgard Riedmaier

  2. Lehrstuhl für Physiologie Wissenschaftszentrum Weihenstephan für Ernährung Landnutzung & Umwelt, Technische Universität München, Weihenstephaner Berg 3, D-85354, Freising-Weihenstephan, Germany

    Michael W. Pfaffl

Authors
  1. Michael W. Pfaffl
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  2. Irmgard Riedmaier
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Corresponding author

Correspondence to Michael W. Pfaffl.

Additional information

Michael W. Pfaffl 1986–1993 Studium der Agrar- und Tierwissenschaften. 1993–1996 Studium der Biotechnologie, 1997 Promotion, 2003 Habilitation (Physiologie) und Privatdozent. Seit 2011 Professor (apl) für Molekulare Physiologie am Lehrstuhl für Physiologie, TU München.

Irmgard Riedmaier 2000–2004 Studium der Biologie (Diplom) an der TU München, 2009 Promotion. Seit 2009 Wissenschaftliche Mitarbeiterin am Lehrstuhl für Physiologie, TU München.

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Pfaffl, M.W., Riedmaier, I. Die Real-Time-RT-PCR-Datenanalyse im Fokus der MIQE-Richtlinie. Biospektrum 17, 295–297 (2011). https://doi.org/10.1007/s12268-011-0047-x

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  • DOI: https://doi.org/10.1007/s12268-011-0047-x

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