Skip to main content
SpringerLink
Log in

Proteinsemisynthese — ein Werkzeug für komplex modifizierte Proteine

  • Wissenschaft · Methoden
  • Proteinchemie
  • Published:
BIOspektrum Aims and scope

Zusammenfassung

Die Proteinsemisynthese ist ein erfolgreiches Beispiel für das Zusammenführen chemischer und biologischer Techniken zur Herstellung modifizierter Proteine. Größte Fortschritte wurden in den letzten Jahren bei der gezielten Einführung posttranslationaler Modifikationen gemacht.

Abstract

Protein semisynthesis is a successful example of combining chemistry and biology to generate modified proteins. Major progress has been made with the specific incorporation of posttranslational modifications over the last years.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Literatur

  1. Dawson PE, Muir TW, Clark-Lewis I et al. (1994) Synthesis of proteins by native chemical ligation. Science 266:776–779

    Article  PubMed  CAS  Google Scholar 

  2. Vila-Perello M, Muir TW (2010) Biological applications of protein splicing. Cell 143:191–200

    Article  PubMed  CAS  Google Scholar 

  3. Mootz HD (2009) Split inteins as versatile tools for protein semisynthesis. Chembiochem 10:2579–2589

    Article  PubMed  CAS  Google Scholar 

  4. Hackenberger CP, Schwarzer D (2008) Chemoselective ligation and modification strategies for peptides and proteins. Angew Chem Int Ed Engl 47:10030–10074

    Article  PubMed  CAS  Google Scholar 

  5. Kee JM, Villani B, Carpenter LR et al. (2010) Development of stable phosphohistidine analogues. J Am Chem Soc 132:14327–14329

    Article  PubMed  CAS  Google Scholar 

  6. Lahiri S, Seidel R, Engelhard M et al. (2010) Photocontrol of STAT6 dimerization and translocation. Mol Biosyst 6:2423–2429

    Article  PubMed  CAS  Google Scholar 

  7. Wu YW, Oesterlin LK, Tan KT et al. (2010) Membrane targeting mechanism of Rab GTPases elucidated by semisynthetic protein probes. Nat Chem Biol 6:534–540

    Article  PubMed  CAS  Google Scholar 

  8. Becker CF, Liu X, Olschewski D et al. (2008) Semisynthesis of a glycosylphosphatidylinositol-anchored prion protein. Angew Chem Int Ed Engl 47:8215–8219

    Article  PubMed  CAS  Google Scholar 

  9. Piontek C, Varon SD, Heinlein C et al. (2009) Semisynthesis of a homogeneous glycoprotein enzyme: ribonuclease C: part 2. Angew Chem Int Ed Engl 48:1941–1945

    Article  PubMed  CAS  Google Scholar 

  10. Martin LJ, Raines RT (2010) Carpe diubiquitin. Angew Chem Int Ed Engl 49:9042–9044

    Article  PubMed  CAS  Google Scholar 

  11. Olschewski D, Seidel R, Miesbauer M et al. (2007) Semisynthetic murine prion protein equipped with a GPI anchor mimic incorporates into cellular membranes. Chem Biol 14:994–1006

    Article  PubMed  CAS  Google Scholar 

  12. Chu NK, Olschewski D, Seidel R et al. (2010) Protein immobilization on liposomes and lipid-coated nanoparticles by protein trans-splicing. J Pept Sci 16:582–588

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Center For Integrated Protein Science Munich, Tu MÜnchen, Germany

    Christian F. W. Becker

  2. Department Chemie AG Proteinchemie, Technische Universität München, Lichtenbergstraße 4, D-85747, Garching b. München, Germany

    Christian F. W. Becker

Authors
  1. Christian F. W. Becker
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Christian F. W. Becker.

Additional information

Christian F. W. Becker Jahrgang 1973. Chemiestudium in Dortmund. 2001 Promotion; 2002–2003 Postdoc bei Gryphon Therapeutics, So. San Francisco, CA, USA. 2004–2007 Arbeitsgruppenleiter am Max-Planck-Institut für molekulare Physiologie, Dortmund. Seit 2007 Professor für Proteinchemie an der Fakultät Chemie der TU München und Mitglied des Center for Integrated Protein Science Munich.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Becker, C.F.W. Proteinsemisynthese — ein Werkzeug für komplex modifizierte Proteine. Biospektrum 17, 291–293 (2011). https://doi.org/10.1007/s12268-011-0046-y

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12268-011-0046-y

Search

Navigation