Zusammenfassung
In einem Forschungsprojekt wurde mit einem Laborroboter aus Blut Gesamt-RNA isoliert, daraus cDNA synthetisiert und diese als Template-DNA zur HLA-Sequenzierung verwendet. Die Ergebnisse wurden mit aktuellen Methoden der HLA-Typisierung verglichen.
Abstract
In a recent research project a laboratory robot was used to isolate total RNA from blood. The cDNA was synthesized and used as a template for HLA sequencing. The results of this sequencing-based HLA typing were compared to state-of-art techniques.
This is a preview of subscription content, log in via an institution to check access.
Literatur
Dunn PPJ, Day S, Williams S et al. (2003) HLA sequencing as a tissue typing tool. In: Goulding N, Seward C (Hrsg) Pediatric Hematology. Humana Press. 233–246
Hammond L, Dunn PPJ (2008) A novel HLA-B*44 allele, HLA-B*4459, identified in a renal patient with aberrant B44 serology and characterized by sequence-based typing. Tissue Antigens 72:494–495
Dunn PPJ, Hammond L, Street J et al. A ’silent’ nucleotide substitution in exon 4 is responsible for the lack of expression of HLA-A*0134N through alternative splicing. Human Immunol (im Druck) Marrean
Author information
Authors and Affiliations
Corresponding authors
Additional information
Marrean Theseira und Paul Dunn
Rights and permissions
About this article
Cite this article
Theseira, M., Dunn, P. & Ziebolz, B. RNA-Isolierung aus Blut für die HLA-Typisierung mittels Sequenzierung. Biospektrum 17, 203–205 (2011). https://doi.org/10.1007/s12268-011-0033-3
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12268-011-0033-3