Abstract
The diagnostic role of T-cell immunoglobulin and mucin domain 3 (Tim-3) expression levels in circulating monocytes in coronary artery disease (CAD) remains to be determined. Here, we enrolled of 265 patients and isolated circulating monocytes from the blood of all participants. We found that the Tim-3 expression levels in monocytes were lower in CAD patients than in the control group. Spearman correlation analysis verified that the Tim-3 levels in monocytes were negatively correlated with the Gensini score and the number of coronary vessels. Multivariate logistic regression analysis showed that the Tim-3 levels in circulating monocytes were negatively correlated with CAD, severe CAD, and three-vessel CAD. The ROC curve showed that Tim-3 possessed high diagnostic value for CAD, severe CAD, and three-vessel CAD, with CAD prediction being the most significant of these values. In conclusion, Tim-3 in circulating monocytes is a novel biomarker for CAD.
Graphical Abstract
T-cell immunoglobulin and mucin domain 3 (Tim-3) in circulating monocytes as a novel biomarker for coronary artery disease.
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Data Availability
Data is available upon request.
Abbreviations
- Tim-3:
-
T-cell immunoglobulin and mucin domain 3
- CAD:
-
Coronary artery disease
- AS:
-
Atherosclerosis
- WBCC:
-
White blood cell count
- NEUT%:
-
Neutrophilic granulocyte percentage
- LYMP%:
-
Lymphocyte proportion
- MONO%:
-
Monocyte percentage
- Cr:
-
Creatinine
- ALT:
-
Alanine aminotransferase
- AST:
-
Aspartate aminotransferase
- LDH:
-
Lactic dehydrogenase
- TC:
-
Total cholesterol
- TG:
-
Triglyceride
- LDL-C:
-
Low-density lipoprotein-cholesterol
- HDL-C:
-
High-density lipoprotein-cholesterol
- Fg:
-
Fibrinogen
- FDP:
-
Fibrinogen degradation products
- DD:
-
D-dimer
- AT-III:
-
Antithrombin III
- APTT:
-
Activated partial thromboplastin time
- qPCR:
-
Quantitative real-time PCR
- GAPDH:
-
Glyceraldehyde 3-phosphate dehydrogenase
- ROC:
-
Receiver operating characteristic
- AUC:
-
Area under the curve
- BMI:
-
Body mass index
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Funding
This work was supported by the Health Commission of Jiangsu Province (grant number ZD2022016) and the Science and Technology Office of Nanjing Medical University (grant number NMU20210039).
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M.H were responsible for designing the experiments. FPX, ZQJ, and L.W. performed the experiments. M.L., XXC and Z.G. collected data. All authors contributed to manuscript preparation and revision.
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The study was approved by the Ethics Committee of the Second Affiliated Hospital of Nanjing Medical University (approval no. 2022-KY-123–01). All patients signed a written informed consent before participating in the study.
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12265_2023_10466_MOESM2_ESM.tif
Supplementary file2 Fig. S1. Correlation analysis of Tim-3 expression in circulating monocytes. a Association between Tim-3 expression and TG. b Association between Tim-3 expression and TC. c Association between Tim-3 expression and LDL-C. D Association between Tim-3 expression and Fg. e Association between Tim-3 expression and DD. f Association between Tim-3 expression and APTT. g Association between Tim-3 expression and PT. h Association between Tim-3 expression and INR. i Association between Tim-3 expression and Gensini score. TC: Total cholesterol; TG: Triglyceride; LDL-C: Low-density lipoprotein-cholesterol; Fg: Fibrinogen; DD: D-dimer; APTT: Activated partial thromboplastin time; PT: Prothrombin time; INR: International normalized ratio (TIF 8717 KB)
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Xiao, F., Jia, Z., Wang, L. et al. T-cell Immunoglobulin and Mucin Domain 3 in Circulating Monocytes as a Novel Biomarker for Coronary Artery Disease. J. of Cardiovasc. Trans. Res. (2023). https://doi.org/10.1007/s12265-023-10466-7
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DOI: https://doi.org/10.1007/s12265-023-10466-7