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Danlou Tablet Protects Against Myocardial Infarction Through Promoting eNOS-Dependent Endothelial Protection and Angiogenesis

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Abstract

Myocardial infarction (MI) is one of the leading causes of death worldwide. Danlou tablet (Dan) is an effective traditional Chinese medicine for cardiac protection, although the underlying mechanism was not fully understood. In this study, we used a murine MI model and demonstrated that Dan administration effectively attenuated myocardial apoptosis, cardiac remodeling, and heart failure post MI. Dan increased CD31-positive capillaries in MI hearts, and reduced the apoptosis and oxidative stress in human umbilical vein endothelial cells after oxygen–glucose deprivation stress, simultaneously with the activated HIF-1α/VEGFA/eNOS signaling. Moreover, inhibition of eNOS by L-NAME attenuated Dan-induced protection against MI, and abolished its effect in promoting angiogenesis and reducing endothelial apoptosis and oxidative stress. Collectively, Dan is beneficial to promote eNOS-dependent endothelial protection and angiogenesis thus protecting against MI. A deep understanding of Dan-induced protection might help promote clinical usage of Dan in MI treatment.

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All data generated or analysed during this study are included in this article and its supplementary material files.

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Acknowledgements

This work was supported by the grants from National Key Research and Development Program of China (2017YFC1700401 to YH Bei), National Natural Science Foundation of China (81970335 and 82170285 to YH Bei, 82000253 to HY Wang), the Sailing Program from Science and Technology Commission of Shanghai (20YF1414000 to HY Wang), the grant from Science and Technology Commission of Shanghai Municipality (23010500300 and 21SQBS00100 to YH Bei, 23ZR1422900 to HY Wang), and “Chenguang Program” of Shanghai Education Development Foundation and Shanghai Municipal Education Commission (20CG46 to HY Wang).

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  1. Yujiao Zhu, Yibo Chai, Zhuhua Su contributed equally to this work.

Authors and Affiliations

  1. School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, China

    Yujiao Zhu & Zheng Jiao

  2. Cardiac Regeneration and Ageing Lab, Institute of Geriatrics (Shanghai University), Affiliated Nantong Hospital of Shanghai University (The Sixth People’s Hospital of Nantong), School of Medicine, Shanghai University, Nantong, 226011, China

    Yujiao Zhu, Yibo Chai, Zhuhua Su, Weitong Qi, Mingming Yin, Lin Li, Meng Wei, Jun Ge, Hongyun Wang & Yihua Bei

  3. Shanghai Engineering Research Center of Organ Repair, School of Life Science, Shanghai University, Shanghai, 200444, China

    Yibo Chai, Zhuhua Su, Weitong Qi, Mingming Yin, Lin Li, Meng Wei, Jun Ge, Hongyun Wang & Yihua Bei

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  1. Yujiao Zhu
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Contributions

Y Zhu, Y Chai, Z Su, W Qi, M Yin, L Li, M Wei, and J Ge performed the experiments and analyzed the data. Y Bei and H Wang designed the study, instructed the experiments, and drafted the manuscript. Z Jiao provided technical support and instructed the experiments for the work. All authors read and approved the final manuscript.

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Correspondence to Hongyun Wang, Zheng Jiao or Yihua Bei.

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Zhu, Y., Chai, Y., Su, Z. et al. Danlou Tablet Protects Against Myocardial Infarction Through Promoting eNOS-Dependent Endothelial Protection and Angiogenesis. J. of Cardiovasc. Trans. Res. 17, 403–416 (2024). https://doi.org/10.1007/s12265-023-10437-y

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