Transverse aortic constriction (TAC) in mice is the most popular model to mimic pressure overload heart disease. In this study, we developed a convenient, quick, and less invasive new TAC mice model. Briefly, after anesthetization, endotracheal intubation was then performed, and the endotracheal tube was connected to a ventilator. The second intercostal space was opened and then the home-made retractors were used to push aside the thymus gently. A tunnel under the aortic arch was made and a segment of 6–0 monofilament polypropylene suture which had been threaded through a specifically modified blunted 26-gauge syringe needle was passed through the tunnel. A blunted 27-gauge needle was placed parallel to the transverse aorta and then three knots were tied quickly. After ligation, the spacer was removed promptly and gently to achieve a constriction of 0.4 mm in diameter. Five weeks after TAC, cardiac hypertrophy, fibrosis, and left ventricular dysfunction were observed.
The mouse was anesthetized with pentobarbital (50 mg/kg) via intraperitoneal injection. Endotracheal intubation under direct vision was then performed and the endotracheal tube was connected to a ventilator. The second intercostal space was opened and then the home-made retractors were used to push aside the thymus gently. A tunnel under the aortic arch was made and a segment of 6–0 monofilament polypropylene suture which had been threaded through a specifically modified blunted 26-gauge syringe needle was passed through the tunnel.
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Transverse aortic constriction
Left common carotid artery
LV end-diastolic internal diameter
LV end-systolic internal diameter
Interventricular septum end-diastolic thickness
Interventricular septum end-systolic thickness
LV end-diastolic posterior wall thickness
LV end-systolic posterior wall thickness
LV end-diastolic volume
LV end-systolic volume
LV ejection fraction
LV fractional shortening
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This study was funded by the National Natural Science Foundation of China (Grant No. 81770319).
No human studies were carried out by the authors for this article.
Ethics Approval for Use of Animals
All institutional and national guidelines for the care and use of laboratory animals were followed and approved by the Institutional Animal Use and Care Committee of Sun Yat-sen University (2019001114).
Conflict of Interest
The authors declare no competing interests.
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Huayang Li and Quan Liu contributed equally to this article.
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Li, H., Liu, Q., Wang, S. et al. A New Minimally Invasive Method of Transverse Aortic Constriction in Mice. J. of Cardiovasc. Trans. Res. (2021). https://doi.org/10.1007/s12265-021-10170-4
- Transverse aortic constriction