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Targeting the MicroRNA-490-3p-ATG4B-Autophagy Axis Relieves Myocardial Injury in Ischemia Reperfusion

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Abstract

We investigated the potential role of miR-490-3p in ischemia reperfusion (IR) injury. We first determined the expression of miR-490-3p and autophagy-related 4B cysteine (ATG4B) in IR. Then, to explore whether miR-490-3p would affect autophagy, apoptosis, and IR injury, we evaluated apoptosis, autophagy, and infarct size via gain- and loss-of-function experiments. Furthermore, we used adenovirus to enhance or inhibit the expression of ATG4B, and then measured autophagy, apoptosis, and IR injury. miR-490-3p was downregulated in the hearts during the process of IR, while ATG4B was upregulated. The inhibition of miR-490-3p or overexpression of ATG4B could promote the expression of LC3II, increase the autolysosomes, inhibit the expression of p62, and reduce infarct size. On all accounts, the inhibition of miR-490-3p could promote autophagy to reduce myocardial IR injury by upregulating ATG4B, a finding that provides new insights for the protective mechanism of autophagy in IR.

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Acknowledgments

We would like to offer our sincere appreciation to the reviewers for their critical comments on this article.

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Data sharing not applicable to this article as no datasets were generated or analyzed during the current study.

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Authors and Affiliations

  1. Department of Cardiology, Liuzhou Traditional Chinese Medical Hospita, The Third Affiliated Hospital of Guangxi University of Chinese Medicine, Liuzhou, 545001, People’s Republic of China

    Yufu Wu

  2. Department of Cardiology, Nanjing Lishui People’s Hospital, Zhongda Hospital Lishui Branch, Southeast University, No. 86, Chongwen Road, Lishui District, Nanjing, 211200, Jiangsu, People’s Republic of China

    Qing Mao

  3. Department of Neurology, The Second Affiliated Hospital of Guangxi Medical University, Nanning, 530007, People’s Republic of China

    Xiulin Liang

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  1. Yufu Wu
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  2. Qing Mao
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Contributions

Yufu Wu and Qing Mao designed the study. Xiulin Liang collated the data. Yufu Wu, Qing Mao, and Xiulin Liang contributed to drafting the manuscript. All authors have read and approved the final submitted manuscript.

Corresponding author

Correspondence to Qing Mao.

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Ethics Statement

The protocol of animal experiments was approved by the Institutional Animal Care and Use Committee of Nanjing Lishui People’s Hospital, Zhongda Hospital Lishui Branch, Southeast University.

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The authors declare that they have no conflicts of interest.

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Associate Editor Joost Sluijter oversaw the review of this article

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Wu, Y., Mao, Q. & Liang, X. Targeting the MicroRNA-490-3p-ATG4B-Autophagy Axis Relieves Myocardial Injury in Ischemia Reperfusion. J. of Cardiovasc. Trans. Res. 14, 173–183 (2021). https://doi.org/10.1007/s12265-020-09972-9

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  • DOI: https://doi.org/10.1007/s12265-020-09972-9

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