Reduced Myocardial Mitochondrial ROS Production in Mechanically Unloaded Hearts
- 33 Downloads
Abstract
Mechanical ventricular unloading in advanced heart failure (HF) has been shown to induce reverse remodeling in myocardial tissues. Little is known about the impact of ventricular unloading on myocardial energy metabolism. We hypothesized that left ventricular unloading reduces myocardial mitochondrial reactive oxygen species (ROS) production and improves mitochondrial coupling efficiency in patients suffering from advanced HF. Left ventricular tissue specimens were harvested from explanted hearts at the time of transplantation. We compared myocardial metabolism in explanted hearts supported with an unloading ventricular assist device prior to transplantation (LVAD-HTX; n = 9) with tissue specimens of unsupported failing hearts (HTX; n = 6). Myocardial mitochondrial ROS production was decreased by 40% in LVAD-HTX compared to HTX patients (1.5 ± 0.3 vs. 0.9 ± 0.1 pmol/(s/mg); p < 0.05). High-resolution respirometry revealed increased mitochondrial coupling efficiency in LVAD-HTX patients (respiratory/control ratio 1.7 ± 0.2 vs. 1.2 ± 0.2; p < 0.05). In conclusion, ventricular unloading is related to decreased mitochondrial ROS production and increased coupling efficiency in myocardium of human failing hearts, suggesting a novel pathomechanism of unloading-associated cardioprotection.
Keywords
Heart failure Left ventricular unloading Left ventricular assist device Heart transplantation Myocardial energy metabolism High-resolution respirometry Mitochondrial respiration Reactive oxygen speciesReferences
- 1.Dayer, M., & Cowie, M. R. (2004). Heart failure: diagnosis and healthcare burden. Clinical Medicine (London, England), 4(1), 13–18.CrossRefGoogle Scholar
- 2.Braunwald, E. (2013). Heart failure. JACC Heart Fail, 1(1), 1–20.CrossRefPubMedGoogle Scholar
- 3.Ponikowski, P., et al. (2016). ESC Guidelines for the diagnosis and treatment of acute and chronic heart failure: The Task Force for the diagnosis and treatment of acute and chronic heart failure of the European Society of Cardiology (ESC). Developed with the special contribution of the Heart Failure Association (HFA) of the ESC. European Journal of Heart Failure, 18(8), 891–975.CrossRefPubMedGoogle Scholar
- 4.Deng, M. C., et al. (2004). Mechanical circulatory support device database of the International Society for Heart and Lung Transplantation: second annual report—2004. The Journal of Heart and Lung Transplantation, 23(9), 1027–1034.CrossRefPubMedGoogle Scholar
- 5.Burkhoff, D., Klotz, S., & Mancini, D. M. (2006). LVAD-induced reverse remodeling: basic and clinical implications for myocardial recovery. Journal of Cardiac Failure, 12(3), 227–239.CrossRefPubMedGoogle Scholar
- 6.Klotz, S., et al. (2004). Left ventricular pressure and volume unloading during pulsatile versus nonpulsatile left ventricular assist device support. The Annals of Thoracic Surgery, 77(1), 143–9; discussion 149-50.CrossRefPubMedGoogle Scholar
- 7.Ponikowski, P., et al. (2016). ESC Guidelines for the diagnosis and treatment of acute and chronic heart failure: The Task Force for the diagnosis and treatment of acute and chronic heart failure of the European Society of Cardiology (ESC)Developed with the special contribution of the Heart Failure Association (HFA) of the ESC. European Heart Journal, 37(27), 2129–2200.CrossRefPubMedGoogle Scholar
- 8.Levin, H. R., et al. (1995). Reversal of chronic ventricular dilation in patients with end-stage cardiomyopathy by prolonged mechanical unloading. Circulation, 91(11), 2717–2720.CrossRefPubMedGoogle Scholar
- 9.de Jonge, N., et al. (2002). Left ventricular assist device in end-stage heart failure: persistence of structural myocyte damage after unloading. An immunohistochemical analysis of the contractile myofilaments. Journal of the American College of Cardiology, 39(6), 963–969.CrossRefPubMedGoogle Scholar
- 10.Vatta, M., et al. (2002). Molecular remodelling of dystrophin in patients with end-stage cardiomyopathies and reversal in patients on assistance-device therapy. Lancet, 359(9310), 936–941.CrossRefPubMedGoogle Scholar
- 11.Bayeva, M., Gheorghiade, M., & Ardehali, H. (2013). Mitochondria as a therapeutic target in heart failure. Journal of the American College of Cardiology, 61(6), 599–610.CrossRefPubMedGoogle Scholar
- 12.Lee, S. H., et al. (1998). Improvement of myocardial mitochondrial function after hemodynamic support with left ventricular assist devices in patients with heart failure. The Journal of Thoracic and Cardiovascular Surgery, 116(2), 344–349.CrossRefPubMedGoogle Scholar
- 13.Nagoshi, T., et al. (2011). Optimization of cardiac metabolism in heart failure. Current Pharmaceutical Design, 17(35), 3846–3853.CrossRefPubMedPubMedCentralGoogle Scholar
- 14.Holzem, K. M., et al. (2016). Mitochondrial structure and function are not different between nonfailing donor and end-stage failing human hearts. The FASEB Journal, 30(8), 2698–2707.CrossRefPubMedPubMedCentralGoogle Scholar
- 15.Stride, N., et al. (2013). Decreased mitochondrial oxidative phosphorylation capacity in the human heart with left ventricular systolic dysfunction. European Journal of Heart Failure, 15(2), 150–157.CrossRefPubMedGoogle Scholar
- 16.Tsutsui, H., Kinugawa, S., & Matsushima, S. (2011). Oxidative stress and heart failure. American Journal of Physiology. Heart and Circulatory Physiology, 301(6), H2181–H2190.CrossRefPubMedGoogle Scholar
- 17.Mondal, N. K., et al. (2013). Oxidative stress, DNA damage and repair in heart failure patients after implantation of continuous flow left ventricular assist devices. International Journal of Medical Sciences, 10(7), 883–893.CrossRefPubMedPubMedCentralGoogle Scholar
- 18.Templeton, D. L., et al. (2012). Effects of left ventricular assist device (LVAD) placement on myocardial oxidative stress markers. Heart, Lung & Circulation, 21(9), 586–597.CrossRefGoogle Scholar
- 19.Koliaki, C., et al. (2015). Adaptation of hepatic mitochondrial function in humans with non-alcoholic fatty liver is lost in steatohepatitis. Cell Metabolism, 21(5), 739–746.CrossRefPubMedGoogle Scholar
- 20.Szendroedi, J., Phielix, E., & Roden, M. (2011). The role of mitochondria in insulin resistance and type 2 diabetes mellitus. Nature Reviews. Endocrinology, 8(2), 92–103.CrossRefPubMedGoogle Scholar
- 21.Pesta, D., & Gnaiger, E. (2012). High-resolution respirometry: OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods in Molecular Biology, 810, 25–58.CrossRefPubMedGoogle Scholar
- 22.Gnaiger, E. (2008). Polarographic oxygen sensors, the oxygraph, and high-resolution respirometry to assess mitochondrial function. In Drug-induced mitochondrial dysfunction (pp. 325–352).CrossRefGoogle Scholar
- 23.Gnaiger, E. (2009). Capacity of oxidative phosphorylation in human skeletal muscle: new perspectives of mitochondrial physiology. The International Journal of Biochemistry & Cell Biology, 41(10), 1837–1845.CrossRefGoogle Scholar
- 24.Krumschnabel, G., et al. (2015). Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods in Molecular Biology, 1264, 245–261.CrossRefPubMedGoogle Scholar
- 25.Makrecka-Kuka, M., Krumschnabel, G., & Gnaiger, E. (2015). High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules, 5(3), 1319–1338.CrossRefPubMedPubMedCentralGoogle Scholar
- 26.Fleckenstein-Elsen, M., et al. (2016). Eicosapentaenoic acid and arachidonic acid differentially regulate adipogenesis, acquisition of a brite phenotype and mitochondrial function in primary human adipocytes. Molecular Nutrition & Food Research, 60(9), 2065–2075.CrossRefGoogle Scholar
- 27.Larsen, S., et al. (2012). Biomarkers of mitochondrial content in skeletal muscle of healthy young human subjects. The Journal of Physiology, 590(14), 3349–3360.CrossRefPubMedPubMedCentralGoogle Scholar
- 28.Lemieux, H., et al. (2011). Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. The International Journal of Biochemistry & Cell Biology, 43(12), 1729–1738.CrossRefGoogle Scholar
- 29.Dipla, K., et al. (1998). Myocyte recovery after mechanical circulatory support in humans with end-stage heart failure. Circulation, 97(23), 2316–2322.CrossRefPubMedGoogle Scholar
- 30.Heerdt, P. M., et al. (2002). Disease-specific remodeling of cardiac mitochondria after a left ventricular assist device. The Annals of Thoracic Surgery, 73(4), 1216–1221.CrossRefPubMedGoogle Scholar
- 31.Razeghi, P., et al. (2002). Downregulation of metabolic gene expression in failing human heart before and after mechanical unloading. Cardiology, 97(4), 203–209.CrossRefPubMedGoogle Scholar
- 32.Keith, M., et al. (1998). Increased oxidative stress in patients with congestive heart failure 11This study was supported by a grant jointly sponsored by the Medical Research Council of Canada, Ottawa and Bayer pharmaceuticals, Etobicoke, Ontario, Canada. Journal of the American College of Cardiology, 31(6), 1352–1356.CrossRefPubMedGoogle Scholar
- 33.Ide, T., et al. (2001). Mitochondrial DNA damage and dysfunction associated with oxidative stress in failing hearts after myocardial infarction. Circulation Research, 88(5), 529–535.CrossRefPubMedGoogle Scholar
- 34.Sawyer, D. B., et al. (2002). Role of oxidative stress in myocardial hypertrophy and failure. Journal of Molecular and Cellular Cardiology, 34(4), 379–388.CrossRefPubMedGoogle Scholar
- 35.Caruso, R., et al. (2012). Severity of oxidative stress and inflammatory activation in end-stage heart failure patients are unaltered after 1 month of left ventricular mechanical assistance. Cytokine, 59(1), 138–144.CrossRefPubMedGoogle Scholar
- 36.Stanley, B. A., et al. (2011). Thioredoxin reductase-2 is essential for keeping low levels of H(2)O(2) emission from isolated heart mitochondria. The Journal of Biological Chemistry, 286(38), 33669–33677.CrossRefPubMedPubMedCentralGoogle Scholar
- 37.Aon, M. A., et al. (2012). Glutathione/thioredoxin systems modulate mitochondrial H2O2 emission: an experimental-computational study. The Journal of General Physiology, 139(6), 479–491.CrossRefPubMedPubMedCentralGoogle Scholar
- 38.Molina, A. J., et al. (2016). Skeletal muscle mitochondrial content, oxidative capacity, and Mfn2 expression are reduced in older patients with heart failure and preserved ejection fraction and are related to exercise intolerance. JACC Heart Fail, 4(8), 636–645.CrossRefPubMedPubMedCentralGoogle Scholar
- 39.Melenovsky, V., et al. (2017). Myocardial iron content and mitochondrial function in human heart failure: a direct tissue analysis. European Journal of Heart Failure, 19(4), 522–530.CrossRefPubMedGoogle Scholar