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Leukemia Inhibitory Factor Increases Survival of Pluripotent Stem Cell-Derived Cardiomyocytes

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A Correction to this article was published on 14 November 2017

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Abstract

Leukemia inhibitory factor (LIF) is a growth factor with pleiotropic biological functions. It has been reported that LIF acts at different stages during mesoderm development. Also, it has been shown that LIF has a cytoprotective effect on neonatal murine cardiomyocytes (CMs) in culture, but little is known about the role of LIF during human cardiogenesis. Thus, we analyzed the effects of LIF on human pluripotent stem cells (PSC) undergoing cardiac differentiation. We first showed that LIF is expressed in the human heart during early development. We found that the addition of LIF within a precise time window during the in vitro differentiation process significantly increased CMs viability. This finding was associated to a decrease in the expression of pro-apoptotic protein Bax, which coincides with a reduction of the apoptotic rate. Therefore, the addition of LIF may represent a promising strategy for increasing CMs survival derived from PSCs.

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  • 14 November 2017

    Please note that Carolina Blüguermann’s surname was misspelled (as Blugüermann) in this article as originally published.

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Acknowledgements

Special thanks to Dario Fernandez Espinosa for his technical support.

Funding

This study was funded by Fondo para la Investigación Científica y Tecnológica (FONCyT) (PICT-2011-2717).

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Authors and Affiliations

  1. LIAN-CONICET, FLENI - Ruta 9 Km 52.5, Belén de Escobar, Buenos Aires, Argentina

    Carolina Blüguermann, Leonardo Romorini, Ximena Garate, Gabriel Neiman, Gustavo Emilio Sevlever, María Elida Scassa & Santiago Gabriel Miriuka

  2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Autónoma de Buenos Aires, Argentina

    Leonardo Romorini & Santiago Gabriel Miriuka

  3. Hoffman Medical Research Center, Universtiy of Southern California, Los Angeles, CA, USA

    Denis Evseenko

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  1. Carolina Blüguermann
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  2. Leonardo Romorini
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  6. Gustavo Emilio Sevlever
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Correspondence to Santiago Gabriel Miriuka.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Associate Editor Saptarsi Haldar oversaw the review of this article

Please note that Carolina Blüguermann’s surname was misspelled (as Blugüermann) in this article as originally published.

A correction to this article is available online at https://doi.org/10.1007/s12265-017-9771-z.

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Blüguermann, C., Romorini, L., Evseenko, D. et al. Leukemia Inhibitory Factor Increases Survival of Pluripotent Stem Cell-Derived Cardiomyocytes. J. of Cardiovasc. Trans. Res. 11, 1–13 (2018). https://doi.org/10.1007/s12265-017-9769-6

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