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A Systematic Investigation of Complement and Coagulation-Related Protein in Autism Spectrum Disorder Using Multiple Reaction Monitoring Technology

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Abstract

Autism spectrum disorder (ASD) is one of the common neurodevelopmental disorders in children. Its etiology and pathogenesis are poorly understood. Previous studies have suggested potential changes in the complement and coagulation pathways in individuals with ASD. In this study, using multiple reactions monitoring proteomic technology, 16 of the 33 proteins involved in this pathway were identified as differentially-expressed proteins in plasma between children with ASD and controls. Among them, CFHR3, C4BPB, C4BPA, CFH, C9, SERPIND1, C8A, F9, and F11 were found to be altered in the plasma of children with ASD for the first time. SERPIND1 expression was positively correlated with the CARS score. Using the machine learning method, we obtained a panel composed of 12 differentially-expressed proteins with diagnostic potential for ASD. We also reviewed the proteins changed in this pathway in the brain and blood of patients with ASD. The complement and coagulation pathways may be activated in the peripheral blood of children with ASD and play a key role in the pathogenesis of ASD.

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Data Availability

All raw data have been deposited as an online resource to the Figshare database with the name: “A systematic investigation of complement and coagulation-related protein in autism spectrum disorder by multiple reaction monitoring technology” (https://doi.org/10.6084/m9.figshare.21830145.v1).

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31870825), the Shenzhen Bureau of Science, Technology, and Information (JCYJ20170412110026229), the Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions (2022SHIBS0003), and 2019 Guiyang Science and Technology Bureau, and the Guiyang First People’s Hospital, Great Health Science and Technology Cooperation Project. We thank all the individuals who participated in the study and the Instrument Analysis Center of Shenzhen University.

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  1. College of Life Science and Oceanography, Shenzhen University, Shenzhen, 518060, China

    Xueshan Cao, Xiaoxiao Tang, Jing Lin, Huajie Zhang, Qiong Liu, Qihong Zheng, Hongbin Zhuang, Xukun Liu, Naseer Ullah Khan & Liming Shen

  2. College of Physics and Optoelectronics Engineering, Shenzhen University, Shenzhen, 518060, China

    Xueshan Cao & Qiong Liu

  3. Maternal and Child Health Hospital of Baoan, Shenzhen, 518100, China

    Chengyun Feng

  4. Department of Endocrinology, Guiyang First People’s Hospital, Guiyang, 550002, China

    Haiying Li

  5. Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions, Shenzhen, 518055, China

    Liming Shen

  6. Shenzhen Key Laboratory of Marine Biotechnology and Ecology, Shenzhen, 518060, China

    Liming Shen

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This research was approved by the Human Research Ethics Committees of Shenzhen University (M20220203) and the Maternal and Child Health Hospital of Baoan (20170801) and complies with the guidelines of the Helsinki Declaration. Written informed consent for study participation was given by the children’s guardians.

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Cao, X., Tang, X., Feng, C. et al. A Systematic Investigation of Complement and Coagulation-Related Protein in Autism Spectrum Disorder Using Multiple Reaction Monitoring Technology. Neurosci. Bull. 39, 1623–1637 (2023). https://doi.org/10.1007/s12264-023-01055-4

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