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Microglial Depletion does not Affect the Laterality of Mechanical Allodynia in Mice

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A Correction to this article was published on 07 September 2023

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Abstract

Mechanical allodynia (MA), including punctate and dynamic forms, is a common and debilitating symptom suffered by millions of chronic pain patients. Some peripheral injuries result in the development of bilateral MA, while most injuries usually led to unilateral MA. To date, the control of such laterality remains poorly understood. Here, to study the role of microglia in the control of MA laterality, we used genetic strategies to deplete microglia and tested both dynamic and punctate forms of MA in mice. Surprisingly, the depletion of central microglia did not prevent the induction of bilateral dynamic and punctate MA. Moreover, in dorsal root ganglion–dorsal root–sagittal spinal cord slice preparations we recorded the low-threshold Aβ-fiber stimulation-evoked inputs and outputs of superficial dorsal horn neurons. Consistent with behavioral results, microglial depletion did not prevent the opening of bilateral gates for Aβ pathways in the superficial dorsal horn. This study challenges the role of microglia in the control of MA laterality in mice. Future studies are needed to further understand whether the role of microglia in the control of MA laterality is etiology-or species-specific.

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Acknowledgements

We thank Prof. Bo Peng (Fudan University) for helpful discussions and comments on this project. We thank all the members of the Cheng laboratory for their support and discussions. This work was supported by grants from the Ministry of Science and Technology of China (2021ZD0203302), the National Natural Science Foundation of China (32170996 and 32060199), the Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions (2021SHIBS0002), the Guangdong Science and Technology Committee (2019A1515010041, A2021319), the Shenzhen Innovation Committee of Science and Technology (ZDSYS20200811144002008), and the Shenzhen Science and Technology Innovation Committee (JCYJ20180302174233348).

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  1. Quan Ma, Dongmei Su, and Jiantao Huo contributed equally to this work.

Authors and Affiliations

  1. School of Life Science and Technology, Harbin Institute of Technology, Harbin, 150001, China

    Quan Ma

  2. Shenzhen Key Laboratory of Gene Regulation and Systems Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen, 518055, China

    Quan Ma, Dongmei Su, Jiantao Huo, Guangjuan Yin, Dong Dong, Kaifang Duan, Hong Cheng, Dong Liu & Longzhen Cheng

  3. Department of Biology, Brain Research Center, Southern University of Science and Technology, Shenzhen, 518055, China

    Quan Ma, Dongmei Su, Jiantao Huo, Guangjuan Yin, Dong Dong, Kaifang Duan, Hong Cheng, Huiling Xu, Jiao Ma, Dong Liu & Longzhen Cheng

  4. Institute of Life Science, Nanchang University, Nanchang, 330031, China

    Bin Mou & Jiyun Peng

  5. Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions, Shenzhen, 518055, China

    Longzhen Cheng

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Ma, Q., Su, D., Huo, J. et al. Microglial Depletion does not Affect the Laterality of Mechanical Allodynia in Mice. Neurosci. Bull. 39, 1229–1245 (2023). https://doi.org/10.1007/s12264-022-01017-2

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