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MouseVenue3D: A Markerless Three-Dimension Behavioral Tracking System for Matching Two-Photon Brain Imaging in Free-Moving Mice

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Abstract

Understanding the connection between brain and behavior in animals requires precise monitoring of their behaviors in three-dimensional (3-D) space. However, there is no available three-dimensional behavior capture system that focuses on rodents. Here, we present MouseVenue3D, an automated and low-cost system for the efficient capture of 3-D skeleton trajectories in markerless rodents. We improved the most time-consuming step in 3-D behavior capturing by developing an automatic calibration module. Then, we validated this process in behavior recognition tasks, and showed that 3-D behavioral data achieved higher accuracy than 2-D data. Subsequently, MouseVenue3D was combined with fast high-resolution miniature two-photon microscopy for synchronous neural recording and behavioral tracking in the freely-moving mouse. Finally, we successfully decoded spontaneous neuronal activity from the 3-D behavior of mice. Our findings reveal that subtle, spontaneous behavior modules are strongly correlated with spontaneous neuronal activity patterns.

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Acknowledgements

This work was supported by the Key Area R&D Program of Guangdong Province, China (2018B030338001 and 2018B030331001), the National Key R&D Program of China (2018YFA0701403), the National Natural Science Foundation of China (31500861, 31630031, 91732304, and 31930047), Chang Jiang Scholars Program, the International Big Science Program Cultivating Project of the Chinese Academy of Science (CAS; 172644KYS820170004), the Strategic Priority Research Program of the CAS (XDB32030100), the Youth Innovation Promotion Association of the CAS (2017413), the CAS Key Laboratory of Brain Connectome and Manipulation (2019DP173024), Shenzhen Government Basic Research Grants (JCYJ20170411140807570, JCYJ20170413164535041), the Science, Technology and Innovation Commission of Shenzhen Municipality (JCYJ20160429185235132), a Helmholtz-CAS Joint Research grant (GJHZ1508), Guangdong Provincial Key Laboratory of Brain Connectome and Behavior (2017B030301017), the Ten Thousand Talent Program, the Guangdong Special Support Program, Key Laboratory of Shenzhen Institute of Advanced Technology (2019DP173024), and the Shenzhen Key Science and Technology Infrastructure Planning Project (ZDKJ20190204002).

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  1. Yaning Han, Kang Huang, and Ke Chen contributed equally to this work.

Authors and Affiliations

  1. Shenzhen Key Laboratory of Neuropsychiatric Modulation and Collaborative Innovation Center for Brain Science, Guangdong Provincial Key Laboratory of Brain Connectome and Behavior, CAS Center for Excellence in Brain Science and Intelligence Technology, Brain Cognition and Brain Disease Institute, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen–Hong Kong Institute of Brain Science, Shenzhen Fundamental Research Institutions, Shenzhen, 518055, China

    Yaning Han, Kang Huang, Ke Chen, Hongli Pan, Furong Ju, Yueyue Long, Gao Gao, Liping Wang & Pengfei Wei

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Yaning Han, Kang Huang, Ke Chen, Liping Wang & Pengfei Wei

  3. University of Rochester, Rochester, NY, 14627, USA

    Yueyue Long

  4. Honam University, Gwangju, 62399, South Korea

    Gao Gao

  5. State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking University, Beijing, 100101, China

    Runlong Wu

  6. Department of Electronics, Peking University, Beijing, 100871, China

    Aimin Wang

  7. State Key Laboratory of Advanced Optical Communication Systems and Networks, Peking University, Beijing, 100101, China

    Aimin Wang

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  1. Yaning Han
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Han, Y., Huang, K., Chen, K. et al. MouseVenue3D: A Markerless Three-Dimension Behavioral Tracking System for Matching Two-Photon Brain Imaging in Free-Moving Mice. Neurosci. Bull. 38, 303–317 (2022). https://doi.org/10.1007/s12264-021-00778-6

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