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Synchrotron Radiation-Based Three-Dimensional Visualization of Angioarchitectural Remodeling in Hippocampus of Epileptic Rats

  • Pan Gu
  • Zi-Hao Xu
  • Yu-Ze Cao
  • Sheng-Hui Liao
  • Qian-Fang Deng
  • Xian-Zhen Yin
  • Zhuo-Lu Wang
  • Zhuo-Hui Chen
  • Xin-Hang Hu
  • Hui Wang
  • Li-Zhi Li
  • Shi-Xin Liu
  • Hui Ding
  • Shu-Peng Shi
  • Hong-Lei Li
  • Ti-Qiao Xiao
  • Bo XiaoEmail author
  • Meng-Qi ZhangEmail author
Original Article
  • 68 Downloads

Abstract

Characterizing the three-dimensional (3D) morphological alterations of microvessels under both normal and seizure conditions is crucial for a better understanding of epilepsy. However, conventional imaging techniques cannot detect microvessels on micron/sub-micron scales without angiography. In this study, synchrotron radiation (SR)-based X-ray in-line phase-contrast imaging (ILPCI) and quantitative 3D characterization were used to acquire high-resolution, high-contrast images of rat brain tissue under both normal and seizure conditions. The number of blood microvessels was markedly increased on days 1 and 14, but decreased on day 60 after seizures. The surface area, diameter distribution, mean tortuosity, and number of bifurcations and network segments also showed similar trends. These pathological changes were confirmed by histological tests. Thus, SR-based ILPCI provides systematic and detailed views of cerebrovascular anatomy at the micron level without using contrast-enhancing agents. This holds considerable promise for better diagnosis and understanding of the pathogenesis and development of epilepsy.

Keywords

Epilepsy Synchrotron radiation 3D Angioarchitecture Blood vessel Remodeling 

Notes

Acknowledgements

This work was completed at the BL13W1 beamline of the Shanghai Synchrotron Radiation Facility (SSRF) in China and was supported by Key Research Project of the Ministry of Science and Technology of China (2016YFC0904400), the National Natural Science Foundation of China (81501025 & 81671299), the Natural Science Foundation of Hunan Province (2016JJ3174), and the Science and Technology Department Funds of Hunan Province Key Project (2016JC2057). We would like to thank Prof. Tiqiao Xiao and other staff at the BL13W1 station of SSRF for their kind assistance during the experiments.

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Copyright information

© Shanghai Institutes for Biological Sciences, CAS 2019

Authors and Affiliations

  • Pan Gu
    • 1
  • Zi-Hao Xu
    • 1
  • Yu-Ze Cao
    • 1
  • Sheng-Hui Liao
    • 2
  • Qian-Fang Deng
    • 1
  • Xian-Zhen Yin
    • 3
  • Zhuo-Lu Wang
    • 4
  • Zhuo-Hui Chen
    • 1
  • Xin-Hang Hu
    • 1
  • Hui Wang
    • 1
  • Li-Zhi Li
    • 1
  • Shi-Xin Liu
    • 1
  • Hui Ding
    • 1
  • Shu-Peng Shi
    • 1
  • Hong-Lei Li
    • 1
  • Ti-Qiao Xiao
    • 5
  • Bo Xiao
    • 1
    Email author
  • Meng-Qi Zhang
    • 1
    Email author
  1. 1.Department of Neurology, Xiangya HospitalCentral South UniversityChangshaChina
  2. 2.School of Information Science and EngineeringCentral South UniversityChangshaChina
  3. 3.State Key Laboratory of Drug Research, CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghaiChina
  4. 4.Department of Breast SurgeryHunan Provincial Maternal and Child Health Care HospitalChangshaChina
  5. 5.Shanghai Synchrotron Radiation Facility, Shanghai Advanced Research InstituteChinese Academy of SciencesShanghaiChina

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