Regional Metabolic Patterns of Abnormal Postoperative Behavioral Performance in Aged Mice Assessed by 1H-NMR Dynamic Mapping Method
Abnormal postoperative neurobehavioral performance (APNP) is a common phenomenon in the early postoperative period. The disturbed homeostatic status of metabolites in the brain after anesthesia and surgery might make a significant contribution to APNP. The dynamic changes of metabolites in different brain regions after anesthesia and surgery, as well as their potential association with APNP are still not well understood. Here, we used a battery of behavioral tests to assess the effects of laparotomy under isoflurane anesthesia in aged mice, and investigated the metabolites in 12 different sub-regions of the brain at different time points using proton nuclear magnetic resonance (1H-NMR) spectroscopy. The abnormal neurobehavioral performance occurred at 6 h and/or 9 h, and recovered at 24 h after anesthesia/surgery. Compared with the control group, the altered metabolite of the model group at 6 h was aspartate (Asp), and the difference was mainly displayed in the cortex; while significant changes at 9 h occurred predominantly in the cortex and hippocampus, and the corresponding metabolites were Asp and glutamate (Glu). All changes returned to baseline at 24 h. The altered metabolic changes could have occurred as a result of the acute APNP, and the metabolites Asp and Glu in the cortex and hippocampus could provide preliminary evidence for understanding the APNP process.
KeywordsAbnormal postoperative neurobehavioral performance 1H-NMR Metabolite Aspartate Glutamate
We would like to express our gratitude to Mrs. Pingping An (Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences) for her help in housing the animals. This work was supported by grants from the National Natural Science Foundation of China (8187051484, 8157050329, and 81600933), the Interdisciplinary Medicine Seed Fund of Peking University (BMU2017MC006), and the Youth Innovation Promotion Association of the Chinese Academy of Sciences, China (Y6Y0021004).
Conflict of interest
The authors declare no competing financial interests.
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