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Increased CXCL13 and CXCR5 in Anterior Cingulate Cortex Contributes to Neuropathic Pain-Related Conditioned Place Aversion

  • Xiao-Bo Wu
  • Li-Na He
  • Bao-Chun Jiang
  • Xue Wang
  • Ying Lu
  • Yong-Jing GaoEmail author
Original Article
  • 53 Downloads

Abstract

Pain consists of sensory-discriminative and emotional-affective components. The anterior cingulate cortex (ACC) is a critical brain area in mediating the affective pain. However, the molecular mechanisms involved remain largely unknown. Our recent study indicated that C-X-C motif chemokine 13 (CXCL13) and its sole receptor CXCR5 are involved in sensory sensitization in the spinal cord after spinal nerve ligation (SNL). Whether CXCL13/CXCR5 signaling in the ACC contributes to the pathogenesis of pain-related aversion remains unknown. Here, we showed that SNL increased the CXCL13 level and CXCR5 expression in the ACC after SNL. Knockdown of CXCR5 by microinjection of Cxcr5 shRNA into the ACC did not affect SNL-induced mechanical allodynia but effectively alleviated neuropathic pain-related place avoidance behavior. Furthermore, electrophysiological recording from layer II–III neurons in the ACC showed that SNL increased the frequency and amplitude of spontaneous excitatory postsynaptic currents (sEPSCs), decreased the EPSC paired-pulse ratio, and increased the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor/N-methyl-D-aspartate receptor ratio, indicating enhanced glutamatergic synaptic transmission. Finally, superfusion of CXCL13 onto ACC slices increased the frequency and amplitude of spontaneous EPSCs. Pre-injection of Cxcr5 shRNA into the ACC reduced the increase in glutamatergic synaptic transmission induced by SNL. Collectively, these results suggest that CXCL13/CXCR5 signaling in the ACC is involved in neuropathic pain-related aversion via synaptic potentiation.

Keywords

CXCL13 CXCR5 Anterior cingulate cortex Neuropathic pain Conditioned place aversion Synaptic transmission 

Notes

Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (31671091 and 81771197), the Natural Science Foundation of Jiangsu Province, China (BK20171255), and the Science and Technology Planning Project of Nantong Municipality, China (MS12017023-9).

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Copyright information

© Shanghai Institutes for Biological Sciences, CAS 2019

Authors and Affiliations

  • Xiao-Bo Wu
    • 1
  • Li-Na He
    • 1
  • Bao-Chun Jiang
    • 1
  • Xue Wang
    • 1
  • Ying Lu
    • 2
  • Yong-Jing Gao
    • 1
    • 3
    Email author
  1. 1.Institute of Pain Medicine, Institute of Special Environmental MedicineNantong UniversityNantongChina
  2. 2.Department of Nutrition and Food Hygiene, School of Public HealthNantong UniversityNantongChina
  3. 3.Co-innovation Center of NeuroregenerationNantong UniversityNantongChina

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