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Neuroscience Bulletin

, Volume 35, Issue 1, pp 113–123 | Cite as

Next-Generation Tools to Study Autonomic Regulation In Vivo

  • Snigdha Mukerjee
  • Eric LazartiguesEmail author
Review
  • 109 Downloads

Abstract

The recent development of tools to decipher the intricacies of neural networks has improved our understanding of brain function. Optogenetics allows one to assess the direct outcome of activating a genetically-distinct population of neurons. Neurons are tagged with light-sensitive channels followed by photo-activation with an appropriate wavelength of light to functionally activate or silence them, resulting in quantifiable changes in the periphery. Capturing and manipulating activated neuron ensembles, is a recently-designed technique to permanently label activated neurons responsible for a physiological function and manipulate them. On the other hand, neurons can be transfected with genetically-encoded Ca2+ indicators to capture the interplay between them that modulates autonomic end-points or somatic behavior. These techniques work with millisecond temporal precision. In addition, neurons can be manipulated chronically to simulate physiological aberrations by transfecting designer G-protein-coupled receptors exclusively activated by designer drugs. In this review, we elaborate on the fundamental concepts and applications of these techniques in research.

Keywords

Autonomic regulation Optogenetics Calcium sensors DREADD 

Notes

Acknowledgements

This review was supported by grants from the National Institutes of Health (HL093178 to EL and CoBRE P30 GM106392) and Louisiana State University Health Sciences Research Enhancement Program.

Conflict of interest

The authors report no conflict of interest.

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Copyright information

© Shanghai Institutes for Biological Sciences, CAS 2018

Authors and Affiliations

  1. 1.Department of Pharmacology and Experimental TherapeuticsLouisiana State University Health Sciences CenterNew OrleansUSA
  2. 2.Neuroscience and Cardiovascular Centers of ExcellenceLouisiana State University Health Sciences CenterNew OrleansUSA
  3. 3.Southeast Louisiana Veterans Health Care SystemNew OrleansUSA

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