Comparison of Reference Genes for Transcriptional Studies in Postmortem Human Brain Tissue Under Different Conditions
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Research on neurodegenerative diseases is a hot topic worldwide , and MRI, genetics and epigenetics, and animal models have been commonly used. Considering species differences, research on human brain samples is irreplaceable . Many countries and regions have built brain banks to collect and store brain tissue from donors.
Samples provided by a brain bank can be used to study synaptic structure, protein, DNA, RNA [3, 4], and lipids to illuminate the pathological mechanisms underlying neurodegenerative diseases. When brain tissue is used for gene expression research, the quantitative real-time polymerase chain reaction (RT-qPCR) is an accurate method, which simultaneously amplifies and quantifies the expression of target genes by measuring the intensity of fluorescence in each PCR cycle; it has the advantages of high efficiency, sensitivity, accuracy, and specificity, along with low cost . However, the value of the PCR cycle threshold requires accurate...
This work was supported by grants from the National Natural Science Foundation of China (81271239, 81771205, and 91632113), the Natural Science Foundation and Major Basic Research Program of Shanghai Municipality, China (16JC1420500 and 16JC1420502), and the CAMS Innovation Fund for Medical Sciences (2017-I2M-3-008). This work was also sponsored by the China Human Brain Bank Consortium.
Conflict of interest
There are no potential conflicts in the financial and material support for this research.
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