Neuroscience Bulletin

, Volume 35, Issue 1, pp 67–78 | Cite as

Angiotensin-Converting Enzyme 2 in the Rostral Ventrolateral Medulla Regulates Cholinergic Signaling and Cardiovascular and Sympathetic Responses in Hypertensive Rats

  • Yu Deng
  • Xing Tan
  • Miao-Ling Li
  • Wei-Zhong Wang
  • Yang-Kai WangEmail author
Original Article


The rostral ventrolateral medulla (RVLM) is a key region in cardiovascular regulation. It has been demonstrated that cholinergic synaptic transmission in the RVLM is enhanced in hypertensive rats. Angiotensin-converting enzyme 2 (ACE2) in the brain plays beneficial roles in cardiovascular function in hypertension. The purpose of this study was to determine the effect of ACE2 overexpression in the RVLM on cholinergic synaptic transmission in spontaneously hypertensive rats (SHRs). Four weeks after injecting lentiviral particles containing enhanced green fluorescent protein and ACE2 bilaterally into the RVLM, the blood pressure and heart rate were notably decreased. ACE2 overexpression significantly reduced the concentration of acetylcholine in microdialysis fluid from the RVLM and blunted the decrease in blood pressure evoked by bilateral injection of atropine into the RVLM in SHRs. In conclusion, we suggest that ACE2 overexpression in the RVLM attenuates the enhanced cholinergic synaptic transmission in SHRs.


Hypertension Renin-angiotensin system Gene transfer Acetylcholine Sympathetic nerve activity 



This work was supported by the National Natural Science Foundation of China (81470534, 81770419, 81630012, and 81570385) and the Key Laboratory of Medical Electrophysiology (Southwest Medical University), Ministry of Education of China - No201709 (KeyME-2017-09).

Compliance with Ethical Standards

Conflict of interest

All authors claim that there are no conflict of interest.


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Copyright information

© Shanghai Institutes for Biological Sciences, CAS and Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Yu Deng
    • 1
    • 3
  • Xing Tan
    • 1
    • 2
  • Miao-Ling Li
    • 4
  • Wei-Zhong Wang
    • 1
    • 2
  • Yang-Kai Wang
    • 1
    • 2
    Email author
  1. 1.Department of PhysiologySecond Military Medical UniversityShanghaiChina
  2. 2.Institution of Polar Medicine Research CenterSecond Military Medical UniversityShanghaiChina
  3. 3.Department of AnesthesiologyChanghai HospitalShanghaiChina
  4. 4.Institute of Cardiovascular Medical ResearchSouthwest Medical UniversityLuzhouChina

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