Cortical Inflammation is Increased in a DSS-Induced Colitis Mouse Model
While inflammatory bowel disease (IBD) might be a risk factor in the development of brain dysfunctions, the underlying mechanisms are largely unknown. Here, mice were treated with 5% dextran sodium sulfate (DSS) in drinking water and sacrificed on day 7. The serum level of IL-6 increased, accompanied by elevation of the IL-6 and TNF-α levels in cortical tissue. However, the endotoxin concentration in plasma and brain of mice with DSS-induced colitis showed a rising trend, but with no significant difference. We also found significant activation of microglial cells and reduction in occludin and claudin-5 expression in the brain tissue after DSS-induced colitis. These results suggested that DSS-induced colitis increases systemic inflammation which then results in cortical inflammation via up-regulation of serum cytokines. Here, we provide new information on the impact of colitis on the outcomes of cortical inflammation.
KeywordsCortical inflammation Dextran sodium sulfate Colitis Endotoxin Blood-brain barrier
We thank Prof. TM Zhang of Academy of Military Medical Sciences for helpful comments. This work was supported by grants from the National Natural Science Foundation of China (81430044) and the National Basic Research Development Program of China (2012CB518200 and 2011CB910800).
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Conflict of interest
The authors have no conflict of interest to declare
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