Early Activation of Astrocytes does not Affect Amyloid Plaque Load in an Animal Model of Alzheimer’s Disease
Astrocytes are closely associated with Alzheimer’s disease (AD). However, their precise roles in AD pathogenesis remain controversial. One of the reasons behind the different results reported by different groups might be that astrocytes were targeted at different stages of disease progression. In this study, by crossing hAPP (human amyloid precursor protein)-J20 mice with a line of GFAP-TK mice, we found that astrocytes were activated specifically at an early stage of AD before the occurrence of amyloid plaques, while microglia were not affected by this crossing. Activation of astrocytes at the age of 3–5 months did not affect the proteolytic processing of hAPP and amyloid plaque loads in the brains of hAPP-J20 mice. Our data suggest that early activation of astrocytes does not affect the deposition of amyloid β in an animal model of AD.
KeywordsAstrocyte Alzheimer’s disease Amyloid plaque Mouse
We thank Dr. Edward Koo (Department of Neuroscience, UCSD) for providing the CT-15 antibody. The 3D6 was provided by Janssen Research & Development (South San Francisco, CA). We are grateful to the Core Facilities of Zhejiang University Institute of Neuroscience for technical assistance. This work was supported by grants from the National Basic Research Development Program of China (2014CB964602), the National Natural Science Foundation of China (91132713 and 81400878), the Zhejiang Provincial Natural Science Foundation of China (LR13H090001), the Science and Technology Planning Project of Zhejiang Province, China (2017C03011), the ‘Double First-Rate’ Project Initiative, and Chinese Ministry of Education Project 111 Program (B13026).
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Conflict of interest
The authors disclosed that there was no conflict of interest.
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