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Neuroscience Bulletin

, Volume 34, Issue 6, pp 912–920 | Cite as

Early Activation of Astrocytes does not Affect Amyloid Plaque Load in an Animal Model of Alzheimer’s Disease

  • Dongpi Wang
  • Xiaoqin Zhang
  • Mingkai Wang
  • Dongming Zhou
  • Hongyu Pan
  • Qiang Shu
  • Binggui SunEmail author
Original Article

Abstract

Astrocytes are closely associated with Alzheimer’s disease (AD). However, their precise roles in AD pathogenesis remain controversial. One of the reasons behind the different results reported by different groups might be that astrocytes were targeted at different stages of disease progression. In this study, by crossing hAPP (human amyloid precursor protein)-J20 mice with a line of GFAP-TK mice, we found that astrocytes were activated specifically at an early stage of AD before the occurrence of amyloid plaques, while microglia were not affected by this crossing. Activation of astrocytes at the age of 3–5 months did not affect the proteolytic processing of hAPP and amyloid plaque loads in the brains of hAPP-J20 mice. Our data suggest that early activation of astrocytes does not affect the deposition of amyloid β in an animal model of AD.

Keywords

Astrocyte Alzheimer’s disease Amyloid plaque Mouse 

Notes

Acknowledgments

We thank Dr. Edward Koo (Department of Neuroscience, UCSD) for providing the CT-15 antibody. The 3D6 was provided by Janssen Research & Development (South San Francisco, CA). We are grateful to the Core Facilities of Zhejiang University Institute of Neuroscience for technical assistance. This work was supported by grants from the National Basic Research Development Program of China (2014CB964602), the National Natural Science Foundation of China (91132713 and 81400878), the Zhejiang Provincial Natural Science Foundation of China (LR13H090001), the Science and Technology Planning Project of Zhejiang Province, China (2017C03011), the ‘Double First-Rate’ Project Initiative, and Chinese Ministry of Education Project 111 Program (B13026).

Compliance with Ethical Standards

Conflict of interest

The authors disclosed that there was no conflict of interest.

Supplementary material

12264_2018_262_MOESM1_ESM.pdf (178 kb)
Supplementary material 1 (PDF 178 kb)

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Copyright information

© Shanghai Institutes for Biological Sciences, CAS and Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Dongpi Wang
    • 1
    • 2
    • 3
  • Xiaoqin Zhang
    • 1
    • 2
  • Mingkai Wang
    • 1
    • 2
  • Dongming Zhou
    • 1
    • 2
    • 3
  • Hongyu Pan
    • 1
    • 2
  • Qiang Shu
    • 3
  • Binggui Sun
    • 1
    • 2
    Email author
  1. 1.Department of Neurobiology, NHC and CAMS Key Laboratory of Medical NeurobiologyZhejiang University School of MedicineHangzhouChina
  2. 2.Department of Neurology of the First Affiliated Hospital, NHC and CAMS Key Laboratory of Medical NeurobiologyZhejiang University School of MedicineHangzhouChina
  3. 3.Children’s HospitalZhejiang University School of MedicineHangzhouChina

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