Rat Cerebrospinal Fluid Treatment Method through Cisterna Cerebellomedullaris Injection
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Drugs that lack the ability to cross the blood-brain barrier (BBB) need to be placed directly into the central nervous system. Our laboratory studies the involvement of the glutamatergic system in the aggressiveness of glioma, and some ligands of glutamate receptors cannot permeate the BBB. Here, glioma-implanted rats were treated by a technique that delivers ligands directly into the cerebrospinal fluid by puncture into the cisterna cerebellomedullaris. Rats were anesthetized and fixed in a rodent stereotactic device. The head was gently tilted downwards at an angle that allowed exposure of the cisterna. Injection into the cisterna was done freehand using a gingival needle coupled to a microsyringe. The efficiency of intracisternal injection was demonstrated using a methylene blue solution. This type of injection is adaptable for any rodent model using small volumes of a variety of other drugs, and is an interesting method for neuroscience studies.
KeywordsNeurobiology Neuroscience Drug administration Intracisternal injection Cisterna magna Cerebrospinal fluid treatment Central nervous system Surgical technique Rodent
This work was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) – Edital Doenças Neurodegenerativas, Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS), and Financiadora de Estados e Projetos (FINEP).
Compliance with Ethical Standards
Conflict of interest
All authors claim that there are no conflicts of interest.
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