Neuroscience Bulletin

, Volume 29, Issue 4, pp 484–492 | Cite as

Valproic acid reduces autophagy and promotes functional recovery after spinal cord injury in rats

  • Hai-Hu Hao
  • Li Wang
  • Zhi-Jian Guo
  • Lang Bai
  • Rui-Ping Zhang
  • Wei-Bing Shuang
  • Yi-Jia Jia
  • Jie Wang
  • Xiao-Yu Li
  • Qiang Liu
Original Article

Abstract

Secondary damage is a critical determinant of the functional outcome in patients with spinal cord injury (SCI), and involves multiple mechanisms of which the most important is the loss of nerve cells mediated by multiple factors. Autophagy can result in cell death, and plays a key role in the development of SCI. It has been recognized that valproic acid (VPA) is neuroprotective in certain experimental animal models, however, the levels of autophagic changes in the process of neuroprotection by VPA treatment following SCI are still unknown. In the present study, we determined the extent of autophagy after VPA treatment in a rat model of SCI. We found that both the mRNA and protein levels of Beclin-1 and LC3 were significantly increased at 1, 2, and 6 h after SCI and peaked at 2 h; however, Western blot showed that autophagy was markedly decreased by VPA treatment at 2 h post-injury. Besides, post-SCI treatment with VPA improved the Basso-Beattie-Bresnahan scale, increased the number of ventral horn motoneurons, and reduced myelin sheath damage compared with vehicle-treated animals at 42 days after SCI. Together, our results demonstrated the characteristics of autophagy expression following SCI, and found that VPA reduced autophagy and enhanced motor function.

Keywords

spinal cord injury autophagy valproic acid LC3 Beclin-1 

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Copyright information

© Shanghai Institutes for Biological Sciences, CAS and Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Hai-Hu Hao
    • 1
  • Li Wang
    • 2
  • Zhi-Jian Guo
    • 3
  • Lang Bai
    • 3
  • Rui-Ping Zhang
    • 4
  • Wei-Bing Shuang
    • 5
  • Yi-Jia Jia
    • 1
  • Jie Wang
    • 2
  • Xiao-Yu Li
    • 2
  • Qiang Liu
    • 6
  1. 1.Department of OrthopaedicsaDayi Hospital of Shanxi Medical UniversityTaiyuanChina
  2. 2.Department of PhysiologyShanxi Medical UniversityTaiyuanChina
  3. 3.Department of OrthopaedicsTaiyuan City Centre HospitalTaiyuanChina
  4. 4.Department of RadiologyFirst Hospital of Shanxi Medical UniversityTaiyuanChina
  5. 5.Department of UrologyFirst Hospital of Shanxi Medical UniversityTaiyuanChina
  6. 6.Department of OrthopaedicsaDayi Hospital of Shanxi Medical UniversityTaiyuanChina

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