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Chromophore-assisted laser inactivation in neural development

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Abstract

Chromophore-assisted laser inactivation (CALI) is a technique that uses photochemically-generated reactive oxygen species to acutely inactivate target proteins in living cells. Neural development includes highly dynamic cellular processes such as asymmetric cell division, migration, axon and dendrite outgrowth and synaptogenesis. Although many key molecules of neural development have been identified since the past decades, their spatiotemporal contributions to these cellular events are not well understood. CALI provides an appealing tool for elucidating the precise functions of these molecules during neural development. In this review, we summarize the principles of CALI, a recent microscopic setup to perform CALI experiments, and the application of CALI to the study of growth-cone motility and neuroblast asymmetric division.

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Authors and Affiliations

  1. National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China

    Wei Li & Guangshuo Ou

  2. The Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, 94158, USA

    Nico Stuurman

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  1. Wei Li
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  2. Nico Stuurman
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  3. Guangshuo Ou
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Correspondence to Guangshuo Ou.

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Li, W., Stuurman, N. & Ou, G. Chromophore-assisted laser inactivation in neural development. Neurosci. Bull. 28, 333–341 (2012). https://doi.org/10.1007/s12264-012-1252-4

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  • DOI: https://doi.org/10.1007/s12264-012-1252-4

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