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Intracellular and current source density analysis of pretectal input to the optic tectum of the frog

青蛙前视盖与视顶盖间神经传导的细胞内记录和电流源密度分析

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Abstract

Objective

Electrophysiological examination of the ipsilateral pretectotectal projection has proved that pretectal cells elicit strong suppressive responses to the ipsilateral tectum. However, the neural mechanisms underlying the contralateral pretectotectal prejection are still obscure. The present study aimed to examine the synaptic nature of pretectal nuclei and contralateral tectal cells, and to demonstrate the spatiotemporal pattern of neuronal activity in the 2 main brain structures.

Methods

Intracellular recording and current source density (CSD) analysis were used to test the complexity of neuronal mechanism of pretectotectal information transfer.

Results

The pretectal stimulation elicited only one type of response on the contralateral tectum, the inhibitory postsynaptic potential (IPSP). The majority of contra-induced IPSPs were assumed to be polysynaptically driven. In the CSD analysis, only one sink with short latency was observed in each profile. The ipsilateral projection produced a prominent monosynaptic sink in layer 8 of tectum. Recipient neurons were located in layers 6 and 7 of tectum. The result confirmed former findings from ipsilateral intracellular recordings.

Conclusion

These results suggest the following neuronal circuit: afferents from the pretectal nuclei broadly inhibit both tectal neuron, and since no second sink occurs in tectal layers, the pretectotectal excitatory afferents probably do not extend over the whole tectum, but are within limited state. The results of intracellular recording and CSD analysis further provide evidence of how pretectal afferent activity flows within the tectal laminae.

摘要

目的

电生理研究已经证实青蛙前视盖对同侧视顶盖细胞有强烈的抑制作用, 但其与对侧视顶盖间神经活动的特性目前尚不清楚。 本研究旨在探讨青蛙前视盖与视顶盖之间复杂的神经活动机理, 并对这种神经活动的时空方式进行分析。

方法

用细胞内记录方法, 通过电刺激前视盖的神经细胞核来记录对侧视顶盖细胞的神经活动。 通过电流源密度分析方法解析细胞外记录的视顶盖细胞的神经活动。

结果

前视盖的电刺激只引起了对侧视顶盖的一种应答, 即抑制性突触后电位(inhibitory postsynaptic potential, IPSP), 且大多数的IPSPs 是通过多突触方式进行神 经信息传递的。 此外, 电流源密度分析结果显示, 前视盖刺激在同侧视顶盖上唤起了非常微弱的兴奋性神经活动, 这与以前的细胞内研究结果相一致。

结论

前视盖的神经细胞能强烈抑制双侧视顶盖细胞的神经活动。 细胞内记录 和电流源密度分析结果有助于进一步揭示前视盖传入在视顶盖层的传导情况。

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Authors and Affiliations

  1. Graduate School of Life Science of Systems Engineering, Department of Brain Science and Engineering, Kyushu Institute of Technology, Fukuoka, 808-0196, Japan

    Xiao-Hong Li  (李晓红), Hong-Jian Kang  (康宏建) & Nobuyoshi Mastumoto  (松本修文)

  2. Department of Ear-Nose-Throat, The First Hospital of Zibo, Zibo, 255200, China

    Xiao-Hong Li  (李晓红) & Mao-Lin Xu  (徐茂林)

  3. Department of Neurosurgery, The First Hospital of Zibo, Zibo, 255200, China

    Hong-Jian Kang  (康宏建)

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  1. Xiao-Hong Li  (李晓红)
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  2. Hong-Jian Kang  (康宏建)
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  3. Mao-Lin Xu  (徐茂林)
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  4. Nobuyoshi Mastumoto  (松本修文)
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Correspondence to Xiao-Hong Li  (李晓红).

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Li, XH., Kang, HJ., Xu, ML. et al. Intracellular and current source density analysis of pretectal input to the optic tectum of the frog. Neurosci. Bull. 26, 371–380 (2010). https://doi.org/10.1007/s12264-010-0520-4

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  • DOI: https://doi.org/10.1007/s12264-010-0520-4

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