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Central administration of Orphanin FQ inhibits GnRH secretion by ORL1 receptor in the median eminence of freely moving ovariectomized rats

中枢注射孤啡肽通过孤儿受体可抑制去卵巢大鼠正中隆起GnRH 释放

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Abstract

Objective

This study aimed to investigate the possible role of Orphanin FQ (OFQ) in the regulation of hypothalamic gonadotropin-releasing hormone (GnRH) secretion.

Methods

The method of push-pull perfusion and radioimmunoassay (RIA) were adopted to examine the secretory profile of GnRH in the median eminence (ME) in freely moving ovariectomized (OVX) rats after intracerebroventricular (icv) injection of OFQ and/or [Nphe1]NC(1–13)NH2 (NC13), a competitive antagonists of the opioid receptor-like 1 receptor (ORL1 receptor).

Results

GnRH release from ME significantly decreased from 40 min to 80 min after the administration of 20 and 200 nmol OFQ in OVX rats (P< 0.05). This inhibitory effect of 20 nmol OFQ could be abolished by pretreatment with equal dose of NC13. More interestingly, GnRH secretion from ME was increased markedly 60 min after icv injection of 100 and 200 nmol NC13 (P < 0.05).

Conclusion

Our results suggested central administration of OFQ could inhibit the release of GnRH in the ME of hypothalamus through ORL1 receptor, providing further in vivo evidence supporting the role of OFQ in the control of GnRH secretion.

摘要

目的

本文旨在研究孤啡肽 (Orphanin FQ, OFQ) 对下丘脑促性腺激素释放激素 (gonadotropin-releasing hormone, GnRH) 释放的调节机制。

方法

应用推挽灌流和放射性免疫测定方法观察侧脑室注射OFQ和/或孤儿受体 (opioid receptor-like1 receptor, ORL1 receptor) 特异性拮抗剂NC13对清醒去卵巢 (ovariectomized, OVX)大鼠下丘脑正中隆起 (median eminence, ME) GnRH 释放的影响。

结果

侧脑室给予20 和200 nmol孤啡肽, 注射后40 到80 min 可明显抑 制OVX大鼠ME的GnRH释放 (P < 0.05), 该作用可被侧脑室注射等剂量NC13预处理所阻断。 侧脑室单独给予100 和200 nmol NC13, 注射后60 min 可使OVX大鼠ME的GnRH释放显著增加 (P < 0.05)。

结论

中枢注射OFQ通过 ORL1 受体可抑制OVX大鼠ME的GnRH释放, 提示下丘脑OFQ可能参与了GnRH释放的生理调节机制。

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Authors and Affiliations

  1. Department of Endocrinology, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, 210029, China

    Xiao-Fei An  (安晓飞) & Jiang-Yi Yu  (余江毅)

  2. Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai, 200032, China

    Ming He  (贺明) & Hao Feng  (冯昊)

  3. Department of Neurobiology and Integrative Medicine, Shanghai Medical College, Fudan University, Shanghai, 200032, China

    Xiao-Fei An  (安晓飞) & Yi Feng  (冯异)

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  1. Xiao-Fei An  (安晓飞)
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  2. Ming He  (贺明)
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  3. Yi Feng  (冯异)
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Correspondence to Xiao-Fei An  (安晓飞).

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An, XF., He, M., Feng, Y. et al. Central administration of Orphanin FQ inhibits GnRH secretion by ORL1 receptor in the median eminence of freely moving ovariectomized rats. Neurosci. Bull. 25, 1–6 (2009). https://doi.org/10.1007/s12264-009-1119-5

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  • DOI: https://doi.org/10.1007/s12264-009-1119-5

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