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Experimental Acute Pancreatitis-Induced Lung Injury—Prevented with “Cytoflavin®”

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Abstract

Severe acute pancreatitis represents about 20% of all pancreatitis diagnosis and it has a high mortality rate when associated with a lung dysfunction. The aim of this research was to investigate the use of a wild spectrum metabolic drug (Cytoflavin®) for the treatment of acute lung injury in experimental pancreatitis. A l-arginine-induced acute pancreatitis (groups II and III) was simulated experimentally in a rat population. A combined metabolic drug was used as treatment. We determined the levels of amylase, medium molecules (MM 254 and 280), malonic aldehyde (MA), diene conjugates (DC), interleukin-8 (IL-8), interleukin-10 (IL-10), and tumor necrosis factor-α (TNF-α) in the blood and we performed a histologic examination of the lungs and pancreas of the rat population. The activation of lipid peroxidation peaks at 24–48 h from the onset of the disease. The level of malonic aldehyde in groups II and III was higher (39.09% and 30.99%, respectively) than that in the control group I (p < 0.02; p < 0.01), the diene conjugates level was higher by 43.66% and 42.03% respectively (p < 0.01). With medical correction after 72 h (group III), the level of malonic aldehyde and diene conjugates decreased by 17.0% and 30.5% when compared with group II (no medical correction) (p < 0.05). The level of medium molecules peaked after 72 h of the disease induction (p < 0.001). A correlation was established between the level of endogenous intoxication with the membrane-destructive processes in the lung tissue 48 h after modeling of acute experimental pancreatitis. The use of the proposed medicament correction reduces the manifestations of endogenous intoxication.

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Author notes

  1. Mariana Fedorkiv and Marco V. Marino contributed equally like first co-authors

Authors and Affiliations

  1. Department of General Surgery, Ivano-Frankivsk National Medical University, 91 Fedkovycha str., Ivano-Frankvisk, 76000, Ukraine

    Mariana Fedorkiv, Roman Kuzenko, Mykola Bahrii, Sergiy Gvozdyk & Galyna Shabat

  2. Department of General and Emergency Surgery, Azienda Ospedaliera, Ospedali Riuniti Villa Sofia-Cervello, Palermo, Italy

    Marco V. Marino

  3. Department of General Surgery, Hospital Universitario Marques de Valdecilla, Santander, Spain

    Marco V. Marino

  4. Institute of Pathology and Cytology, Ruppiner Klinikum, University Hospital of the Brandenburg Medical School, Neuruppin, Germany

    Mykola Bahrii

  5. Casa di Cura Sant’Anna, 34, S. Anna str., 91016, Trapani, Erice, Italy

    Galyna Shabat

Authors
  1. Mariana Fedorkiv
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  2. Marco V. Marino
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  3. Roman Kuzenko
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  4. Mykola Bahrii
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  5. Sergiy Gvozdyk
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Contributions

Mariana Fedorkiv and Marco V. Marino analyzed the data and drafted the article; Mariana Fedorkiv, Roman Kuzenko, and Sergiy Gvozdyk participated in the experiment simulation and data collection; Mykola Bagrii performed histological exams, histological photography, and data collection; Galyna Shabat performed study design and manuscript revision. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Galyna Shabat.

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Ethical Approval

The study was approved by the Institutional Ethical Committee of Ivano-Frankivsk National Medical University (Ivano-Frankivsk, Ukraine) and performed according to the Helsinki Declaration guidelines. (Directives EU № 609 from 24/11/1986 and the Law of Ukraine No. 3447-1 from 21/02/2006).

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The authors declare that they have no conflict of interest.

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Fedorkiv, M., Marino, M.V., Kuzenko, R. et al. Experimental Acute Pancreatitis-Induced Lung Injury—Prevented with “Cytoflavin®”. Indian J Surg 83, 720–725 (2021). https://doi.org/10.1007/s12262-020-02475-6

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  • DOI: https://doi.org/10.1007/s12262-020-02475-6

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