Abstract
Breast carcinoma is the most common cause of carcinoma death in women. Sometimes, difficulty arises to differentiate between premalignant lesions and carcinoma by routine histopathology. Our study was done to establish the role of morphometry and immunohistochemistry to solve this problem. In this study, total 60 cases of different breast lesions were included and 10 controls were also included to compare the results with the normal findings. They were studied by hematoxylin and eosin-stained sections for morphometry and routine histological study; as well as by proliferative markers such as proliferating cell nuclear antigen and p53. Invasiveness was studied using immunohistochemical staining with 34 βE12 monoclonal antibody. Statistically significant differences were found in morphometric parameters and in expression of proliferative markers between most of them. Morphometry and immunohistochemistry help in the proper diagnosis of different breast lesions that lie in the gray zone on routine histopathology.
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Abbreviations
- H&E:
-
Hematoxylin and Eosin
- ME cell:
-
Myoepithelial cell
- DCIS:
-
Ductal carcinoma in situ
- LCIS:
-
Lobular carcinoma in situ
- IDC:
-
Invasive ductal carcinoma
- ILC:
-
Invasive lobular carcinoma
- IHC:
-
Immunohistochemistry
- UDH:
-
Usual ductal hyperplasia
- ADH:
-
Atypical ductal hyperplasia
- PCNA:
-
Proliferating cell nuclear antigen
- MNA:
-
Mean nuclear area
- MNP:
-
Mean nuclear perimeter
- MMND:
-
Mean maximum nuclear diameter
- Mmnd:
-
Mean minimal nuclear diameter
- MND:
-
Mean nuclear diameter
- MCA:
-
Mean cell area
- MCD:
-
Mean cell diameter
- N/C ratio:
-
Nuclear to cytoplasmic ratio
- MAb:
-
Monoclonal antibody
- PCNA LI (%):
-
PCNA labeling index
- μ:
-
micron
- PAP:
-
Peroxidase antiperoxidase
- SD:
-
Standard deviation
- No.:
-
Number
References
Rosai J (2004) Rosai and Ackerman’s surgical pathology. Elsevier, New Delhi, p 1787
Yeole BB, Kurkure AP (2003) An epidemiological assessment of increasing incidence and trends in breast cancer in Mumbai and other sites in India, during the last two decades. Asian Pac J Cancer Prev 4:51–56
Manna AK, Pathak S, Sarkar DK (2010) Role of proliferative markers in breast lesions. Indian J Surg 72:53–57
Radwan MM, Amer KA, Mokhtar NM, Kandil MA, EL-Barbary AM, Aiad HA (2003) Nuclear morphometry in ductal breast carcinoma with correlation to cell proliferative activity and prognosis. J Egypt Nat Cancer Inst 15:169–182
Yeh IT, Mies C (2007) Application of Immunohistochemistry to breast lesions. Arch Pathol LabMed 132:349–358
Mahjoub S, Zahraei M, Karami F, Mohagheghi MA, Zeraeti H (1999) Overexpression of p53 protein in malignant breast tumors: an immunohistochemical study. Acta Medica Iranica 37:5–10
Kumar V, Abbas AK, Fausto N (2004) Robbins and Cotran pathologic basis of disease. Elsevier, Noida, p 1134
Elledge RM, Fuqua SA, Clark GM, Pujol P, Allred DC, McGuire WL (1993) Prognostic significance of p53 gene alterations in node-negative breast cancer. Breast Cancer Res Treat 26:225–235
Leonardi E, Girlando S, Serio G, Mauri FA, Perrone G, Scampini S, Dalla Palma P, Barbareschi M (1992) PCNA and Ki-67 expression in breast carcinoma: correlations with clinical and biological variables. J Clin Pathol 45:416–419
Joshi MG, Lee AK, Pedersen CA, Schnitt S, Camus MG, Hughes KS (1996) The role of immunocytochemical markers in the differential diagnosis of proliferative and neoplastic lesions of the breast. Mod Pathol 9:57–62
Bancroft JD, Gamble M (2008) Theory and practical of histological techniques. Elsevier, China, p 126
Bancroft JD, Gamble M (2008) Theory and practical of histological techniques. Elsevier, China, pp 456–459
van der Kooy K, Rookus MA, Peterse HL, van Leeuwen (1996) p53 Protein overexpression in relation to risk factors for breast cancer. Am J Epidemiol 144:924–933
Ikpatt OF, Kupio T, Collan Y (2002) Nuclear morphometry in African breast cancer. Image Anal Stereol 2:145–150
Skjorten F, Kaaresen R, Jacobsen U, Skaane P, Amilie E (1991) Nuclear morphometry of benign and malignant breast lesions. Eur J Surg Oncol 17:350–353
Ruiz A, Almenar S, Callaghan RC, Llombart-Bosch A (1999) Benign, preinvassive and invasive ductal breast lesions. A comparative study with quantitative techniques: morphometry, image and flow cytometry. Pathol Res Pract 195:741–746
Tanaka T, Masashikimura MK et al (1991) Centrosomal kinase A1K1 is over expressed in invasive ductal carcinomas of the breast. Cancer Res 59:2041–2044
Agarwal S, Jain R, Rusia U, Gupta RL (1997) Proliferating cell nuclear antigen immunostaining in breast carcinoma and its relationship to clinical and pathological variables. Indian J Pathol Microbiol 40:11–16
de a Robbins BA, Vega D, Ogata K (1987) Immunohistochemical detection of proliferating cell nuclear antigen in solid human malignancies. Arch Pathol Lab Med 111:841–845
Song HS, Do YR, Kang SH, Jeong KY, Kim YS (2006) Prognostic significance of immunohistochemical expression of p53 gene product in operable breast cancer. Cancer Res Treat 38:218–223
Koutselini H, Malliri A, Field JK, Spandidos DA (1991) P53 expression in cytologic specimens from benign and malignant breast lesions. Anticancer Res 11:1415–1420
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Manna, A.K., Samaddar, A., Mitra, S. et al. Morphometric and Histological Study of Breast Lesions with Special Reference to Proliferative Activity and Invasiveness. Indian J Surg 75, 204–209 (2013). https://doi.org/10.1007/s12262-012-0486-8
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DOI: https://doi.org/10.1007/s12262-012-0486-8