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Ultrasound-assisted Extraction of Polyphenols from Carthamus tinctorius Seeds: Optimization of Process Variables

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An Erratum to this article was published on 24 December 2022

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Abstract

In this study, optimization of ultrasound-assisted extraction (UAE) conditions was conducted to increase the production of bioactive components from Carthamus tinctorius seeds (CS) using statistically-based optimization. The effects of major independent variables including extraction time (5.0–55.0 min), extraction temperature (26.0–94.0°C), and ethanol concentration (0.0–99.5%) were optimized using central composite design (CCD) to explore conditions that simultaneously maximize total polyphenol content, total flavonoid content, and radical scavenging activity. According to quadratic regression models, the predicted optimum UAE conditions were 33.7 min, 75.1°C, and 68.3% ethanol, respectively. Under these conditions, the experimental results were found to be 9.94 mg gallic acid equivalents/g dry matter (DM) of TPC, 0.28 mg quercetin equivalents/g DM of TFC, and 66.6% of RSA which are in good agreement with the predicted values. CS extract was subjected to LC/MS/MS analysis to analyze components with bioactive activities, and apigenin was determined as the main component. Therefore, we have concluded that UAE is an effective process for extracting antioxidant components and CS extract with bioactive components can be utilized as food, cosmetics, and pharmaceutical materials.

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Authors and Affiliations

  1. Department of Food Science, Sun Moon University, Asan, 31460, Korea

    Jun Hee Kim, Suh Hee Yeom, Youn Seon Hwang, So Hee Kim & Jin Woo Kim

  2. Center for Next-Generation Semiconductor Technology, Sun Moon University, Asan, 31460, Korea

    Jin Woo Kim

  3. FlexPro Biotechnology, Sun Moon University, Asan, 31460, Korea

    Jin Woo Kim

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Kim, J.H., Yeom, S.H., Hwang, Y.S. et al. Ultrasound-assisted Extraction of Polyphenols from Carthamus tinctorius Seeds: Optimization of Process Variables. Biotechnol Bioproc E 27, 869–878 (2022). https://doi.org/10.1007/s12257-022-0092-y

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