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Efficient Immobilization of Enzymes on Amino Functionalized MIL-125-NH2 Metal Organic Framework

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Abstract

As a series of metal organic framework (MOFs), materials of institute lavoisier frameworks (MILs) are expected to be excellent supports for enzyme immobilization due to their good water-stability and acid tolerance. However, enzyme loading on MIL-125-NH2 is very low due to small pore size and few amino groups on the surface of the MIL-125-NH2. In this work, catalase (CAT) was immobilized on the MIL-125-NH2 and amino functionalized MIL-125-NH2 by adsorption (CAT@MIL-125-NH2) and covalent binding (CAT@Amino MIL-125-NH2), respectively. Compared with the CAT@MIL-125-NH2 and free CAT, the CAT@Amino MIL-125-NH2 displayed high activity recovery, good pH stability, stability against denaturants, and thermostability. Furthermore, activity recovery of CAT@Amino MIL-125-NH2 was 56% higher than CAT@MIL-125-NH2. The CAT@Amino MIL-125-NH2 still retained 50% residual activity for 14 days at room temperature, whereas free CAT lost activity after storage for 1 day at the same storage conditions. Furthermore, the CAT@Amino MIL-125-NH2 maintained 62% of its initial activity after 4 consecutive uses, showing good reusability. The results showed that the amino functionalized MIL-125-NH2 is an excellent carrier of enzyme immobilization.

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Acknowledgments

This work is partially supported by the Science and Technology Program of Tianjin, China (project no. 20ZYJDJC00080), the Key Projects of Tianjin Natural Science Foundation, China (project no. 19JCZDJC38100), Key R & D projects in Zhongning County, China (project no. 2021YBYF0808), project of Guangxi Key Research and Development program, China (project no. GuikeAB21238005), and project of Key Research and Development of Shandong province, China (project no. 2021CXGC010509).

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  1. The first and fourth authors contributed equally to this paper.

Authors and Affiliations

  1. State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin, 300457, China

    Zichen Wang, Yang Liu, Jinhong Li, Jiandong Cui & Shiru Jia

  2. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China

    Zichen Wang, Yang Liu, Jinhong Li, Jiandong Cui & Shiru Jia

  3. College of agriculture and Bioengineering, Heze University, Heze city, 274015, China

    Guoqing Meng & Daoyu Zhu

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  1. Zichen Wang
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Wang, Z., Liu, Y., Li, J. et al. Efficient Immobilization of Enzymes on Amino Functionalized MIL-125-NH2 Metal Organic Framework. Biotechnol Bioproc E 27, 135–144 (2022). https://doi.org/10.1007/s12257-020-0393-y

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