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Oxygen Uptake Rate Controlling Strategy Balanced with Oxygen Supply for Improving Coenzyme Q10 Production by Rhodobacter sphaeroides

  • Research Paper
  • Bioprocess Engineering
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Abstract

The effects of different oxygen uptake rates (OUR) on the physiological metabolism of Rhodobacter sphaeroides were investigated systematically in 50 L fermenters due to the significant influence on industrial coenzyme Q10 production under oxygen supply limitation. Meanwhile, the seriously decreased oxygen transfer rate caused by the increased broth viscosity was successfully prevented with OUR-directed continuous ammonium sulfate feeding in the late fermentation phase. The statistical analysis results showed that controlling OUR constantly at 45 ± 2.2 mmol/L/h by the oxygen supply level adjustment and the continuous ammonium sulfate feeding could greatly enhance Q10 production. This OUR-Stat controlling strategy successfully achieved the maximal coenzyme Q10 production (2584 ± 82 mg/L), which was 15.4% higher than that of the control. The highest specific CoQ10 content was 25.9 mg/(g DCW)), and the yield of CoQ10 to glucose consumption was up to 19.37 mg/g. These results demonstrated that the optimal OUR-Stat controlling strategy would be effective and economical for improving the industrial CoQ10 production.

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Acknowledgements

This work was financially supported by the National key research and development program (Grant No. 2017YFF0204602), the Fundamental research funds for the central universities (No. 222201714024), and innovation and intelligence 111 plan.

The authors declare no conflict of interest.

Neither ethical approval nor informed consent was required for this study.

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Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology; Shanghai Institute of Biomanufacturing Technology & Collaborative Innovation Center, Shanghai, 200237, China

    Ze-Jian Wang, Xingzi Zhang, Meijin Guo, Siliang Zhang & Yingping Zhuang

  2. National Institute of Metrology, Beijing, 200237, China

    Ping Wang & Zhiwei Sui

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  1. Ze-Jian Wang
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Wang, ZJ., Zhang, X., Wang, P. et al. Oxygen Uptake Rate Controlling Strategy Balanced with Oxygen Supply for Improving Coenzyme Q10 Production by Rhodobacter sphaeroides. Biotechnol Bioproc E 25, 459–469 (2020). https://doi.org/10.1007/s12257-019-0461-3

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