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In vitro Screening of Traditional Chinese Medicines Compounds Derived with Anti-encephalomyocarditis Virus Activities

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  • Biomedical Engineering
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Abstract

The prevalence of encephalomyocarditis virus has brought about enormous financial losses to the swine industry throughout the globe. Chinese herbal medicines have potential antiviral activity which has been proved. Fifteen traditional Chinese medicine compounds were screened for anti-encephalomyocarditis virus (EMCV) activity. The maximum non-toxic concentration (MNTC), cytotoxic concentration 50% (CC50), maximal inhibition rate (MIR), and effective concentration 50% (EC50) against EMCV were measured using MTT and antiviral assays on baby hamster Syrian kidney (BHK-21) cells. Two of the compounds, baicalin and matrine, with MIR > 50% and selective index (SI) > 3 were chosen for further virus load analysis. The results showed that the MIRs of baicalin and matrine were higher than that of positive control ribavirin while the SI values were much smaller than that of the control. Real-time quantitative PCR analysis demonstrated that baicalin and matrine have significant (p < 0.05) anti-EMCV activity compared to the control. It is a baseline study concluded that baicalin and matrine needs further development as an independent drugs or part of a Chinese medicine prescription for the treatment of EMCV infection.

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Acknowledgements

This work was supported by the National Key R&D Program of China (grant number 2017YFD0501500) and the National Natural Science Foundation of China (grant number 31702285).

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, China

    Jiangang Zheng, Yinlan Xu, Ajab Khan, Hongquan Li & Na Sun

  2. School of Community Health, Faculty of Science, Charles Sturt University, NSW, 2800, Australia

    Shaoyu Wang

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  1. Jiangang Zheng
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These experiments were conducted with compliance with the current laws of China. The authors have no conflict of interest in the research. Neither ethical approval nor informed consent was required for this study.

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Zheng, J., Xu, Y., Khan, A. et al. In vitro Screening of Traditional Chinese Medicines Compounds Derived with Anti-encephalomyocarditis Virus Activities. Biotechnol Bioproc E 25, 181–189 (2020). https://doi.org/10.1007/s12257-019-0354-5

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