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Inhibition of 3T3-L1 Adipocyte Differentiation by D-allulose

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  • Biomedical Engineering
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Abstract

Obesity is a serious problem in modern society and its prevalence continues to increase worldwide, resulting in metabolic disorder related diseases. D-allulose, a sugar substitute, boasts a near-zero calorie value and regulates lipid accumulation. However, the molecular mechanism of D-allulose at the cellular level has not been fully elucidated. In this study, we investigated the effect of D-allulose on 3T3-L1 adipocyte differentiation. D-allulose inhibits differentiation of 3T3-L1 preadipocytes into mature adipocytes, as examined by Oil Red O staining. The mRNA levels of genes involved in lipogenesis, including fatty acid synthase (FAS) and adipocyte fatty acid-binding protein (aP2), were significantly decreased and intracellular triglyceride (TG) content was markedly reduced with D-allulose treatment. We also monitored the activity of major adipogenic transcription factors–CREB, SREBP-1c, and PPARψ–using 3T3-L1 reporter cell lines that were constructed to secrete Gaussia luciferase upon binding of a transcription factor to its DNA binding element. Collectively, D-allulose suppresses adipocyte differentiation and lipid accumulation through regulating adipogenic transcription factors and may have anti-obesity potential.

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Acknowledgements

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. NRF-2017R1A2B1012415).

The authors declare no conflict of interest.

Neither ethical approval nor informed consent was required for this study.

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Authors and Affiliations

  1. Department of Chemical and Material Engineering, The University of Suwon, Hwaseong, 18323, Korea

    Seohyun Moon & Kyungoh Choi

  2. Food Research Institute, CJ CheilJedang, Suwon, 16495, Korea

    Yang Hee Kim

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  1. Seohyun Moon
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Moon, S., Kim, Y.H. & Choi, K. Inhibition of 3T3-L1 Adipocyte Differentiation by D-allulose. Biotechnol Bioproc E 25, 22–28 (2020). https://doi.org/10.1007/s12257-019-0352-7

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